Simian immunodeficiency virus burden in tissues and cellular compartments during clinical latency and AIDS

Todd A. Reinhart; Michael J. Rogan; David Huddleston; Dianne M. Rausch; Lee E. Eiden; Ashley T. Haase

doi:10.1086/514113

Simian immunodeficiency virus burden in tissues and cellular compartments during clinical latency and AIDS

Todd A. Reinhart, Michael J. Rogan, David Huddleston, Dianne M. Rausch, Lee E. Eiden, Ashley T. Haase

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63 Scopus citations

Abstract

In the course of human immunodeficiency virus infection or of the related simian immunodeficiency virus (SIV), progression to AIDS is associated with high virus burdens in blood. How virus burden in the bloodstream is related to virus burden in tissue reservoirs was addressed in an animal model of rhesus macaques infected with SIV. In situ hybridization and quantitative image analysis were used to quantitate virus burden. Animals who developed AIDS had high levels of virus production and storage in lymphoid tissue reservoirs and evidence of productive infection of macrophages in the nervous system. With the quantitative approach described, it should be possible to design and assess the impact of treatment and shed light on the outstanding issues in pathogenesis.

Original language	English
Pages (from-to)	1198-1208
Number of pages	11
Journal	Journal of Infectious Diseases
Volume	176
Issue number	5
DOIs	https://doi.org/10.1086/514113
State	Published - 1997
Externally published	Yes

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10.1086/514113

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@article{62a21bd25e0845c580e3871ad5ca86a2,

title = "Simian immunodeficiency virus burden in tissues and cellular compartments during clinical latency and AIDS",

abstract = "In the course of human immunodeficiency virus infection or of the related simian immunodeficiency virus (SIV), progression to AIDS is associated with high virus burdens in blood. How virus burden in the bloodstream is related to virus burden in tissue reservoirs was addressed in an animal model of rhesus macaques infected with SIV. In situ hybridization and quantitative image analysis were used to quantitate virus burden. Animals who developed AIDS had high levels of virus production and storage in lymphoid tissue reservoirs and evidence of productive infection of macrophages in the nervous system. With the quantitative approach described, it should be possible to design and assess the impact of treatment and shed light on the outstanding issues in pathogenesis.",

author = "Reinhart, {Todd A.} and Rogan, {Michael J.} and David Huddleston and Rausch, {Dianne M.} and Eiden, {Lee E.} and Haase, {Ashley T.}",

note = "Funding Information: Received 2 December 1996; revised 19 March 1997. All animal experiments were done in accordance with National Institutes of Health Guidelines on the Care and Use of Laboratory Animals. Financial support: Pediatric AIDS Foundation Scholar Award (T.A.R.); Volkswagen Foundation (L.E.E.). Reprints or correspondence: Dr. Ashley T. Haase, Dept. of Microbiology, University of Minnesota Medical School, 420 Delaware Street S.E., Minneapolis, MN 55455. * Current address: Immunopathology Unit, Glaxo Wellcome Medicines Research Centre, Gunnels Wood Rd., Stevenage, Herts., SG1 2NY, UK.",

year = "1997",

doi = "10.1086/514113",

language = "English",

volume = "176",

pages = "1198--1208",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "5",

}

TY - JOUR

T1 - Simian immunodeficiency virus burden in tissues and cellular compartments during clinical latency and AIDS

AU - Reinhart, Todd A.

AU - Rogan, Michael J.

AU - Huddleston, David

AU - Rausch, Dianne M.

AU - Eiden, Lee E.

AU - Haase, Ashley T.

N1 - Funding Information: Received 2 December 1996; revised 19 March 1997. All animal experiments were done in accordance with National Institutes of Health Guidelines on the Care and Use of Laboratory Animals. Financial support: Pediatric AIDS Foundation Scholar Award (T.A.R.); Volkswagen Foundation (L.E.E.). Reprints or correspondence: Dr. Ashley T. Haase, Dept. of Microbiology, University of Minnesota Medical School, 420 Delaware Street S.E., Minneapolis, MN 55455. * Current address: Immunopathology Unit, Glaxo Wellcome Medicines Research Centre, Gunnels Wood Rd., Stevenage, Herts., SG1 2NY, UK.

PY - 1997

Y1 - 1997

N2 - In the course of human immunodeficiency virus infection or of the related simian immunodeficiency virus (SIV), progression to AIDS is associated with high virus burdens in blood. How virus burden in the bloodstream is related to virus burden in tissue reservoirs was addressed in an animal model of rhesus macaques infected with SIV. In situ hybridization and quantitative image analysis were used to quantitate virus burden. Animals who developed AIDS had high levels of virus production and storage in lymphoid tissue reservoirs and evidence of productive infection of macrophages in the nervous system. With the quantitative approach described, it should be possible to design and assess the impact of treatment and shed light on the outstanding issues in pathogenesis.

AB - In the course of human immunodeficiency virus infection or of the related simian immunodeficiency virus (SIV), progression to AIDS is associated with high virus burdens in blood. How virus burden in the bloodstream is related to virus burden in tissue reservoirs was addressed in an animal model of rhesus macaques infected with SIV. In situ hybridization and quantitative image analysis were used to quantitate virus burden. Animals who developed AIDS had high levels of virus production and storage in lymphoid tissue reservoirs and evidence of productive infection of macrophages in the nervous system. With the quantitative approach described, it should be possible to design and assess the impact of treatment and shed light on the outstanding issues in pathogenesis.

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U2 - 10.1086/514113

DO - 10.1086/514113

M3 - Article

C2 - 9359719

AN - SCOPUS:0030729690

SN - 0022-1899

VL - 176

SP - 1198

EP - 1208

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 5

ER -

Simian immunodeficiency virus burden in tissues and cellular compartments during clinical latency and AIDS

Abstract

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