TY - JOUR
T1 - Shotgun Lipidomic Profiling of the NCI60 Cell Line Panel Using Rapid Evaporative Ionization Mass Spectrometry
AU - Strittmatter, Nicole
AU - Lovrics, Anna
AU - Sessler, Judit
AU - McKenzie, James S.
AU - Bodai, Zsolt
AU - Doria, M. Luisa
AU - Kucsma, Nora
AU - Szakacs, Gergely
AU - Takats, Zoltan
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/8/2
Y1 - 2016/8/2
N2 - Rapid evaporative ionization mass spectrometry (REIMS) was used for the rapid mass spectrometric profiling of cancer cell lines. Spectral reproducibility was assessed for three different cell lines, and the extent of interclass differences and intraclass variance was found to allow the identification of these cell lines based on the REIMS data. Subsequently, the NCI60 cell line panel was subjected to REIMS analysis, and the resulting data set was investigated for its distinction of individual cell lines and different tissue types of origin. Information content of REIMS spectral profiles of cell lines were found to be similar to those obtained from mammalian tissues although pronounced differences in relative lipid intensity were observed. Ultimately, REIMS was shown to detect changes in lipid content of cell lines due to mycoplasma infection. The data show that REIMS is an attractive means to study cell lines involving minimal sample preparation and analysis times in the range of seconds.
AB - Rapid evaporative ionization mass spectrometry (REIMS) was used for the rapid mass spectrometric profiling of cancer cell lines. Spectral reproducibility was assessed for three different cell lines, and the extent of interclass differences and intraclass variance was found to allow the identification of these cell lines based on the REIMS data. Subsequently, the NCI60 cell line panel was subjected to REIMS analysis, and the resulting data set was investigated for its distinction of individual cell lines and different tissue types of origin. Information content of REIMS spectral profiles of cell lines were found to be similar to those obtained from mammalian tissues although pronounced differences in relative lipid intensity were observed. Ultimately, REIMS was shown to detect changes in lipid content of cell lines due to mycoplasma infection. The data show that REIMS is an attractive means to study cell lines involving minimal sample preparation and analysis times in the range of seconds.
UR - http://www.scopus.com/inward/record.url?scp=84980387476&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.6b00187
DO - 10.1021/acs.analchem.6b00187
M3 - Article
C2 - 27377867
AN - SCOPUS:84980387476
SN - 0003-2700
VL - 88
SP - 7507
EP - 7514
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 15
ER -
