Serological identification and separation of canine lymphocyte subpopulations

E. Holler, H. J. Kolb, U. Bodenberger, R. Eckstein, G. Hoffmann-Fezer, R. P. Linke, W. Mempel, H. Rodt, S. Thierfelder

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2 Scopus citations

Abstract

In vitro treatment of bone marrow grafts with absorbed rabbit-antidog thymocyte globulin (ATG) prevented graft-versus-host disease in a substantial number of the dogs differing by one DLA haplotype. Absorbed ATG has now been used for serological identification of canine lymphocyte populations. Specific labeling of canine T-lymphocytes by absorbed ATG could be demonstrated by (a) a distribution of ATG-positive cells in suspensions of canine lymphoid organs similar to that of T cells observed in other species and by specific staining of paracortical thymucs-dependent lymph node areas in immunohistochemistry, (b) complementary labeling of nylon-wool-separated cells by ATG and antiimmunoglobulin sera, and (c) correlation of ATG surface labeling with responder activities in mixed lymphocyte cultures.

Original languageEnglish
Pages (from-to)774-781
Number of pages8
JournalExperimental Hematology
Volume12
Issue number10
StatePublished - 1984
Externally publishedYes

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