TY - GEN
T1 - Serial sectioning and multispectral imaging system for versatile biomedical applications
AU - Symvoulidis, P.
AU - Cruz Pérez, C.
AU - Schwaiger, M.
AU - Ntziachristos, V.
AU - Westmeyer, G. G.
N1 - Publisher Copyright:
© 2014 IEEE.
PY - 2014/7/29
Y1 - 2014/7/29
N2 - Serial sectioning combined with microscopy provides high resolution volumetric data to complement in-vivo imaging modalities and aid ex-vivo diagnostics. We describe the design of a fully-automated cryomicrotome combined with a multispectral reflection and fluorescence imaging system that enables high-throughput analyses of biological specimens with a large field of view and cellular resolution while keeping the manufacturing and running costs low. We show the performance of the system for representative applications in high-resolution volumetric imaging of reporter animals and multispectral tissue analysis. Furthermore, we demonstrate the versatility of the economical imaging system in applications such as in vivo epifluorescence imaging, histology slide scanning, cell counting and gel electrophoresis documentation.
AB - Serial sectioning combined with microscopy provides high resolution volumetric data to complement in-vivo imaging modalities and aid ex-vivo diagnostics. We describe the design of a fully-automated cryomicrotome combined with a multispectral reflection and fluorescence imaging system that enables high-throughput analyses of biological specimens with a large field of view and cellular resolution while keeping the manufacturing and running costs low. We show the performance of the system for representative applications in high-resolution volumetric imaging of reporter animals and multispectral tissue analysis. Furthermore, we demonstrate the versatility of the economical imaging system in applications such as in vivo epifluorescence imaging, histology slide scanning, cell counting and gel electrophoresis documentation.
KW - 3D visualization
KW - Cryo-microtome serial tissue sectioning
KW - Cryoslicer imaging
KW - Epifluorescence imaging
KW - Multispectral imaging
UR - http://www.scopus.com/inward/record.url?scp=84927944029&partnerID=8YFLogxK
U2 - 10.1109/isbi.2014.6868014
DO - 10.1109/isbi.2014.6868014
M3 - Conference contribution
AN - SCOPUS:84927944029
T3 - 2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014
SP - 890
EP - 893
BT - 2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014
Y2 - 29 April 2014 through 2 May 2014
ER -