TY - JOUR
T1 - Sensitive chemiluminescence immunoassay for the determination of rat serum α1-acid glycoprotein
AU - Metzger, J.
AU - Blobner, M.
AU - Luppa, P. B.
PY - 2001
Y1 - 2001
N2 - We present the establishment of a sensitive immunoassay for the determination of α1-acid glycoprotein (orosomucoid, AGP) in rat serum. The assay is based upon antigen capture by surface-immobilized specific polyclonal rabbit anti-AGP antibodies with biotinylated rat AGP (rAGP) as the tracer, and formatted as competitive enzyme immunoassay. Signaling is performed by streptavidin-conjugated horseradish peroxidase. Enzyme activity is quantified by an enhanced chemiluminometric method, allowing the sensitive detection of rAGP serum levels in small sample volumes.
AB - We present the establishment of a sensitive immunoassay for the determination of α1-acid glycoprotein (orosomucoid, AGP) in rat serum. The assay is based upon antigen capture by surface-immobilized specific polyclonal rabbit anti-AGP antibodies with biotinylated rat AGP (rAGP) as the tracer, and formatted as competitive enzyme immunoassay. Signaling is performed by streptavidin-conjugated horseradish peroxidase. Enzyme activity is quantified by an enhanced chemiluminometric method, allowing the sensitive detection of rAGP serum levels in small sample volumes.
KW - Acute phase reaction
KW - Chemiluminescence immunoassay
KW - Rat α-acid glycoprotein
UR - http://www.scopus.com/inward/record.url?scp=0034921666&partnerID=8YFLogxK
U2 - 10.1515/CCLM.2001.086
DO - 10.1515/CCLM.2001.086
M3 - Article
C2 - 11506464
AN - SCOPUS:0034921666
SN - 1434-6621
VL - 39
SP - 514
EP - 518
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
IS - 6
ER -