Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids

G. Brink; L. Schmitt; R. Tampé; E. Sackmann

doi:10.1016/0005-2736(94)00218-5

Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids

G. Brink
, L. Schmitt
, R. Tampé
, E. Sackmann

Department of Bioscience

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca²⁺-mediated vesicle fusion, confirmed this interpretation.

Original language	English
Pages (from-to)	227-230
Number of pages	4
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1196
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(94)00218-5
State	Published - 30 Dec 1994

Keywords

Near infrared surface plasmon spectroscopy
Supported membrane
Vesicle fusion

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10.1016/0005-2736(94)00218-5

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title = "Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids",

abstract = "We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca2+-mediated vesicle fusion, confirmed this interpretation.",

keywords = "Near infrared surface plasmon spectroscopy, Supported membrane, Vesicle fusion",

author = "G. Brink and L. Schmitt and R. Tamp{\'e} and E. Sackmann",

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T1 - Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids

AU - Brink, G.

AU - Schmitt, L.

AU - Tampé, R.

AU - Sackmann, E.

PY - 1994/12/30

Y1 - 1994/12/30

N2 - We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca2+-mediated vesicle fusion, confirmed this interpretation.

AB - We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca2+-mediated vesicle fusion, confirmed this interpretation.

KW - Near infrared surface plasmon spectroscopy

KW - Supported membrane

KW - Vesicle fusion

UR - https://www.scopus.com/pages/publications/0028606452

U2 - 10.1016/0005-2736(94)00218-5

DO - 10.1016/0005-2736(94)00218-5

M3 - Article

C2 - 7841187

AN - SCOPUS:0028606452

SN - 0005-2736

VL - 1196

SP - 227

EP - 230

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

IS - 2

ER -

Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids

Abstract

Keywords

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