Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids

G. Brink; L. Schmitt; R. Tampé; E. Sackmann

doi:10.1016/0005-2736(94)00218-5

Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids

G. Brink, L. Schmitt, R. Tampé, E. Sackmann

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Technical University of Munich

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41 Scopus citations

Abstract

We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca²⁺-mediated vesicle fusion, confirmed this interpretation.

Original language	English
Pages (from-to)	227-230
Number of pages	4
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1196
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(94)00218-5
State	Published - 30 Dec 1994

Keywords

Near infrared surface plasmon spectroscopy
Supported membrane
Vesicle fusion

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10.1016/0005-2736(94)00218-5

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abstract = "We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca2+-mediated vesicle fusion, confirmed this interpretation.",

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author = "G. Brink and L. Schmitt and R. Tamp{\'e} and E. Sackmann",

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AU - Brink, G.

AU - Schmitt, L.

AU - Tampé, R.

AU - Sackmann, E.

PY - 1994/12/30

Y1 - 1994/12/30

N2 - We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca2+-mediated vesicle fusion, confirmed this interpretation.

AB - We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca2+-mediated vesicle fusion, confirmed this interpretation.

KW - Near infrared surface plasmon spectroscopy

KW - Supported membrane

KW - Vesicle fusion

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DO - 10.1016/0005-2736(94)00218-5

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JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

IS - 2

ER -

Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids

Abstract

Keywords

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