TY - JOUR
T1 - Selective plane illumination optical and optoacoustic microscopy for postembryonic imaging
AU - Lin, Hsiao Chun Amy
AU - Chekkoury, Andrei
AU - Omar, Murad
AU - Schmitt-Manderbach, Tobias
AU - Koberstein-Schwarz, Benno
AU - Mappes, Timo
AU - López-Schier, Hernán
AU - Razansky, Daniel
AU - Ntziachristos, Vasilis
N1 - Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Intravital imaging of large specimens is intrinsically challenging for postembryonic studies. Selective plane illumination microscopy (SPIM) has been introduced to volumetrically visualize organisms used in developmental biology and experimental genetics. Ideally suited for imaging transparent samples, SPIM can offer high frame rate imaging with optical microscopy resolutions and low phototoxicity. However, its performance quickly deteriorates when applied to opaque tissues. To overcome this limitation, SPIM optics were merged with optical and optoacoustic (photoacoustic) readouts. The performance of this hybrid imaging system was characterized using various phantoms and by imaging a highly scattering ex vivo juvenile zebrafish. The results revealed the system's enhanced capability over that of conventional SPIM for high-resolution imaging over extended depths of scattering content. The approach described here may enable future visualization of organisms throughout their entire development, encompassing regimes in which the tissue may become opaque.
AB - Intravital imaging of large specimens is intrinsically challenging for postembryonic studies. Selective plane illumination microscopy (SPIM) has been introduced to volumetrically visualize organisms used in developmental biology and experimental genetics. Ideally suited for imaging transparent samples, SPIM can offer high frame rate imaging with optical microscopy resolutions and low phototoxicity. However, its performance quickly deteriorates when applied to opaque tissues. To overcome this limitation, SPIM optics were merged with optical and optoacoustic (photoacoustic) readouts. The performance of this hybrid imaging system was characterized using various phantoms and by imaging a highly scattering ex vivo juvenile zebrafish. The results revealed the system's enhanced capability over that of conventional SPIM for high-resolution imaging over extended depths of scattering content. The approach described here may enable future visualization of organisms throughout their entire development, encompassing regimes in which the tissue may become opaque.
KW - Biological imaging
KW - Hybrid imaging systems
KW - Light-sheet microscopy
KW - Optoacoustic imaging
UR - http://www.scopus.com/inward/record.url?scp=84942196983&partnerID=8YFLogxK
U2 - 10.1002/lpor.201500120
DO - 10.1002/lpor.201500120
M3 - Article
AN - SCOPUS:84942196983
SN - 1863-8880
VL - 9
SP - L29-L34
JO - Laser and Photonics Reviews
JF - Laser and Photonics Reviews
IS - 5
ER -