Abstract
Minicells derived from E. coli χ796 (F+) are refractory to infection by phage M 13. However, after infection of the minicell-producing strain with M 13, phage DNA is found to segregate efficiently into newly formed minicells. The M 13 specific DNA present in minicells isolated several hours after infection consists of single stranded viral DNA and double stranded replicative forms in nearly equal amounts. M 13 DNA containing minicells are capable of carrying out at least one complete round of single stranded DNA synthesis as shown by the flow of label from replicative forms to free single strands.
Original language | English |
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Pages (from-to) | 203-212 |
Number of pages | 10 |
Journal | MGG Molecular & General Genetics |
Volume | 138 |
Issue number | 3 |
DOIs | |
State | Published - Sep 1975 |
Externally published | Yes |