Screening von myopen LASIK-patienten mit einer gesteigerten epithelialen wundheilung. Quantitative bestimmung von EGF mRNA in den kornealen epithelzellen

Translated title of the contribution: Screening of myopic LASIK patients with increased epithelial wound healing. Quantifying epidermal growth factor mRNA in corneal epithelial cells

Chris P. Lohmann, C. Winkler Von Mohrenfels, U. Reischl, A. Muller, J. L. Guell

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Background. Wound healing of the cornea is critical for the refractive outcome of myopic laser-assisted in situ keratomileusis (LASIK). As epidermal growth factor (EGF) is important for the origin of epithelial hyperplasia, this study examined preoperative EGF mRNA concentrations in the corneal epithelial cells to detect patients with increased epithelial wound healing response. Patients and Methods. The epithelium was biopsied before LASIK in 35 eyes with myopia of-10.0 D. The EGF mRNA concentration in the epithelial cells was quantified by polymerase chain reaction and enzyme-linked oligosorbent assay, and the correlation with postoperative refraction at 6 months was assessed. Results. All eyes were around emmetropia 3 weeks after the surgery. At 6 months postoperatively 27 eyes were within ± 1.0 D of emmetropia while 8 showed regression of 2.0-4.0 D. Higher EGF mRNA levels were found in eyes with regression than in eyes with postoperative emmetropia. Conclusions. Preoperative EGF mRNA concentration in the corneal epithelial cells may be an indicator of postoperative refractive outcome of myopic LASIK and offers a new possibility for pharmaceutical manipulation.

Translated title of the contributionScreening of myopic LASIK patients with increased epithelial wound healing. Quantifying epidermal growth factor mRNA in corneal epithelial cells
Original languageGerman
Pages (from-to)460-465
Number of pages6
JournalOphthalmologe
Volume98
Issue number5
DOIs
StatePublished - May 2001
Externally publishedYes

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