Screening for potential targets to reduce stenosis in bioprosthetic heart valves

Rudi Foth, Orr Shomroni, Matthias Sigler, Jürgen Hörer, Julie Cleuziou, Thomas Paul, Katja Eildermann

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Progressive stenosis is one of the main factors that limit the lifetime of bioprosthetic valved conduits. To improve long-term performance we aimed to identify targets that inhibit pannus formation on conduit walls. From 11 explanted, obstructed, RNAlater presevered pulmonary valved conduits, we dissected the thickened conduit wall and the thin leaflet to determine gene expression-profiles using ultra deep sequencing. Differential gene expression between pannus and leaflet provided the dataset that was screened for potential targets. Promising target candidates were immunohistologically stained to see protein abundance and the expressing cell type(s). While immunostainings for DDR2 and FGFR2 remained inconclusive, EGFR, ErbB4 and FLT4 were specifically expressed in a subset of tissue macrophages, a cell type known to regulate the initiation, maintenance, and resolution of tissue repair. Taken toghether, our data suggest EGFR, ErbB4 and FLT4 as potential target candidates to limit pannus formation in bioprosthestic replacement valves.

Original languageEnglish
Article number2464
JournalScientific Reports
Issue number1
StatePublished - Dec 2021


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