Screening by PCR for Defined DNA Sequences in Minimal Amounts of Barley Tissue

K. J. Dehmer, A. Graner, G. Wenzel

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The potential use of the polymerase chain reaction (PCR) in screening plants for defined DNA sequences was demonstrated in barley (Hordeum vulgare). As templates for PCR, purified DNA as well as DNA from crude cell extracts of different barley tissues can be used. Specific synthesis of the target sequence was possible in both cases. Under optimal conditions, as little as 20 copies of the barley genome, i.e., 20 cells, were sufficient for successful amplification. This amount of DNA is equivalent to significantly less than 1 mg of fresh tissue.

Original languageEnglish
Pages (from-to)70-72
Number of pages3
JournalPlant Breeding
Volume107
Issue number1
DOIs
StatePublished - Aug 1991
Externally publishedYes

Keywords

  • DNA screening
  • Hordeum vulgare
  • PCR
  • selection

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