SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes

HCA Lung Biological Network

doi:10.1038/s41591-020-0868-6

SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes

HCA Lung Biological Network

Chair of Mathematical Modelling of Biological Systems

Wellcome Sanger Institute
UMR 7271
University Medical Center Groningen
Royal Victoria Infirmary
Max Delbrück Center for Molecular Medicine
Harvard Medical School
University of Cambridge
Addenbrooke's Hospital
Cambridge University Hospitals NHS Foundation Trust
University College London
Translational Genomics Research Institute
Wellcome Trust
Stanford University School of Medicine
University of California, San Diego
Rady Children’s Hospital San Diego
University of Colorado Denver School of Medicine
Medizinische Hochschule Hannover
German Center for Infectious Diseases (DZIF)
Department of Neuroscience
University of Washington School of Medicine
Newcastle Hospitals NHS Foundation Trust
Biological Research Centre
University of Helsinki
Partner Site Munich Heart Alliance
Charite Universitätsmedizin Berlin
Charité – Universitätsmedizin Berlin
Massachusetts General Hospital
The Broad Institute of MIT and Harvard
Yale University Medical School
Vanderbilt University Medical Center
Edward Hines VA Medical Center
Vanderbilt School of Medicine
CartaNA AB
Catholic University of Korea
Institut de Pharmacologie Moléculaire et Cellulaire
Karolinska Institutet
Center for Autonomous Systems
Northwestern University
National Heart and Lung Institute
King's College London
Boston Children's Hospital
Harvard Stem Cell Institute
University of Alberta
Weill Cornell Medical College
Garvan Institute of Medical Research
University of New South Wales
Stanford University
Massachusetts General Hospital Cancer Center
Duke University
Howard Hughes Medical Institute
Massachusetts Institute of Technology
Northwestern University Feinberg School of Medicine
Justus-Liebig-Universität Gießen
Helmholtz Zentrum München German Research Center for Environmental Health
Rheinische Friedrich-Wilhelms-Universität Bonn
German Center for Neurodegenerative Diseases (DZNE)
Center for Genes, Environment and Health
National Jewish Health
Brigham and Women's Hospital
Arizona State University
University of Michigan Medical School
University of Michigan, Ann Arbor
Boston University School of Medicine
Johnson & Johnson Innovation Center
Mount Sinai School of Medicine
Jeffrey Cheah Biomedical Centre
Cincinnati Children’s Hospital Medical Center
University of Cincinnati College of Medicine
Universitá di L'Aquila
Zhongshan School of Medicine, SYSU
Department of Bioengineering
University of Pittsburgh

Research output: Contribution to journal › Article › peer-review

2038 Scopus citations

Abstract

We investigated SARS-CoV-2 potential tropism by surveying expression of viral entry-associated genes in single-cell RNA-sequencing data from multiple tissues from healthy human donors. We co-detected these transcripts in specific respiratory, corneal and intestinal epithelial cells, potentially explaining the high efficiency of SARS-CoV-2 transmission. These genes are co-expressed in nasal epithelial cells with genes involved in innate immunity, highlighting the cells’ potential role in initial viral infection, spread and clearance. The study offers a useful resource for further lines of inquiry with valuable clinical samples from COVID-19 patients and we provide our data in a comprehensive, open and user-friendly fashion at www.covid19cellatlas.org.

Original language	English
Pages (from-to)	681-687
Number of pages	7
Journal	Nature Medicine
Volume	26
Issue number	5
DOIs	https://doi.org/10.1038/s41591-020-0868-6
State	Published - 1 May 2020

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SDG 3 Good Health and Well-being

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10.1038/s41591-020-0868-6

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@article{4f67b9d32d4f471cb0bf497763152587,

title = "SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes",

abstract = "We investigated SARS-CoV-2 potential tropism by surveying expression of viral entry-associated genes in single-cell RNA-sequencing data from multiple tissues from healthy human donors. We co-detected these transcripts in specific respiratory, corneal and intestinal epithelial cells, potentially explaining the high efficiency of SARS-CoV-2 transmission. These genes are co-expressed in nasal epithelial cells with genes involved in innate immunity, highlighting the cells{\textquoteright} potential role in initial viral infection, spread and clearance. The study offers a useful resource for further lines of inquiry with valuable clinical samples from COVID-19 patients and we provide our data in a comprehensive, open and user-friendly fashion at www.covid19cellatlas.org.",

author = "\{HCA Lung Biological Network\} and Waradon Sungnak and Ni Huang and Christophe B{\'e}cavin and Marijn Berg and Rachel Queen and Monika Litvinukova and Carlos Talavera-L{\'o}pez and Henrike Maatz and Daniel Reichart and Fotios Sampaziotis and Worlock, \{Kaylee B.\} and Masahiro Yoshida and Barnes, \{Josephine L.\} and Banovich, \{Nicholas E.\} and Pascal Barbry and Alvis Brazma and Joseph Collin and Desai, \{Tushar J.\} and Duong, \{Thu Elizabeth\} and Oliver Eickelberg and Christine Falk and Michael Farzan and Ian Glass and Gupta, \{Ravindra K.\} and Muzlifah Haniffa and Peter Horvath and Norbert Hubner and Deborah Hung and Naftali Kaminski and Mark Krasnow and Kropski, \{Jonathan A.\} and Malte Kuhnemund and Majlinda Lako and Haeock Lee and Sylvie Leroy and Sten Linnarson and Joakim Lundeberg and Meyer, \{Kerstin B.\} and Zhichao Miao and Misharin, \{Alexander V.\} and Nawijn, \{Martijn C.\} and Nikolic, \{Marko Z.\} and Michela Noseda and Jose Ordovas-Montanes and Oudit, \{Gavin Y.\} and Dana Pe{\textquoteright}er and Joseph Powell and Steve Quake and Jay Rajagopal and Theis, \{Fabian J.\}",

note = "Publisher Copyright: {\textcopyright} 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.",

year = "2020",

month = may,

day = "1",

doi = "10.1038/s41591-020-0868-6",

language = "English",

volume = "26",

pages = "681--687",

journal = "Nature Medicine",

issn = "1078-8956",

publisher = "Nature Research",

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TY - JOUR

T1 - SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes

AU - HCA Lung Biological Network

AU - Sungnak, Waradon

AU - Huang, Ni

AU - Bécavin, Christophe

AU - Berg, Marijn

AU - Queen, Rachel

AU - Litvinukova, Monika

AU - Talavera-López, Carlos

AU - Maatz, Henrike

AU - Reichart, Daniel

AU - Sampaziotis, Fotios

AU - Worlock, Kaylee B.

AU - Yoshida, Masahiro

AU - Barnes, Josephine L.

AU - Banovich, Nicholas E.

AU - Barbry, Pascal

AU - Brazma, Alvis

AU - Collin, Joseph

AU - Desai, Tushar J.

AU - Duong, Thu Elizabeth

AU - Eickelberg, Oliver

AU - Falk, Christine

AU - Farzan, Michael

AU - Glass, Ian

AU - Gupta, Ravindra K.

AU - Haniffa, Muzlifah

AU - Horvath, Peter

AU - Hubner, Norbert

AU - Hung, Deborah

AU - Kaminski, Naftali

AU - Krasnow, Mark

AU - Kropski, Jonathan A.

AU - Kuhnemund, Malte

AU - Lako, Majlinda

AU - Lee, Haeock

AU - Leroy, Sylvie

AU - Linnarson, Sten

AU - Lundeberg, Joakim

AU - Meyer, Kerstin B.

AU - Miao, Zhichao

AU - Misharin, Alexander V.

AU - Nawijn, Martijn C.

AU - Nikolic, Marko Z.

AU - Noseda, Michela

AU - Ordovas-Montanes, Jose

AU - Oudit, Gavin Y.

AU - Pe’er, Dana

AU - Powell, Joseph

AU - Quake, Steve

AU - Rajagopal, Jay

AU - Theis, Fabian J.

PY - 2020/5/1

Y1 - 2020/5/1

N2 - We investigated SARS-CoV-2 potential tropism by surveying expression of viral entry-associated genes in single-cell RNA-sequencing data from multiple tissues from healthy human donors. We co-detected these transcripts in specific respiratory, corneal and intestinal epithelial cells, potentially explaining the high efficiency of SARS-CoV-2 transmission. These genes are co-expressed in nasal epithelial cells with genes involved in innate immunity, highlighting the cells’ potential role in initial viral infection, spread and clearance. The study offers a useful resource for further lines of inquiry with valuable clinical samples from COVID-19 patients and we provide our data in a comprehensive, open and user-friendly fashion at www.covid19cellatlas.org.

AB - We investigated SARS-CoV-2 potential tropism by surveying expression of viral entry-associated genes in single-cell RNA-sequencing data from multiple tissues from healthy human donors. We co-detected these transcripts in specific respiratory, corneal and intestinal epithelial cells, potentially explaining the high efficiency of SARS-CoV-2 transmission. These genes are co-expressed in nasal epithelial cells with genes involved in innate immunity, highlighting the cells’ potential role in initial viral infection, spread and clearance. The study offers a useful resource for further lines of inquiry with valuable clinical samples from COVID-19 patients and we provide our data in a comprehensive, open and user-friendly fashion at www.covid19cellatlas.org.

UR - https://www.scopus.com/pages/publications/85083969210

U2 - 10.1038/s41591-020-0868-6

DO - 10.1038/s41591-020-0868-6

M3 - Article

C2 - 32327758

AN - SCOPUS:85083969210

SN - 1078-8956

VL - 26

SP - 681

EP - 687

JO - Nature Medicine

JF - Nature Medicine

IS - 5

ER -

SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes

Abstract

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