SARS-CoV-2 CD8+ T cell killing assays using replicating viruses and transgenic antigens

Laura M. Mateyka; Vincent Grass; Andreas Pichlmair; Dirk H. Busch; Elvira D'Ippolito

doi:10.1016/j.xpro.2022.101699

SARS-CoV-2 CD8⁺ T cell killing assays using replicating viruses and transgenic antigens

Laura M. Mateyka, Vincent Grass, Andreas Pichlmair, Dirk H. Busch, Elvira D'Ippolito

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The quality of an antigen-specific CD8⁺ T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8⁺ T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiological system for antigen presentation. We detail the use of target cells either infected with replicating SARS-CoV-2 virus or engineered with SARS-CoV-2 open reading frames. For complete details on the use and execution of this protocol, please refer to Moosmann et al. (2022) and Wagner et al. (2022).

Original language	English
Article number	101699
Journal	STAR Protocols
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2022.101699
State	Published - 16 Dec 2022

Keywords

CRISPR
Cell Biology
Cell culture
Cell-based Assays
Immunology

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10.1016/j.xpro.2022.101699

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title = "SARS-CoV-2 CD8+ T cell killing assays using replicating viruses and transgenic antigens",

abstract = "The quality of an antigen-specific CD8+ T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8+ T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiological system for antigen presentation. We detail the use of target cells either infected with replicating SARS-CoV-2 virus or engineered with SARS-CoV-2 open reading frames. For complete details on the use and execution of this protocol, please refer to Moosmann et al. (2022) and Wagner et al. (2022).",

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author = "Mateyka, {Laura M.} and Vincent Grass and Andreas Pichlmair and Busch, {Dirk H.} and Elvira D'Ippolito",

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AU - Grass, Vincent

AU - Pichlmair, Andreas

AU - Busch, Dirk H.

AU - D'Ippolito, Elvira

PY - 2022/12/16

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AB - The quality of an antigen-specific CD8+ T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8+ T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiological system for antigen presentation. We detail the use of target cells either infected with replicating SARS-CoV-2 virus or engineered with SARS-CoV-2 open reading frames. For complete details on the use and execution of this protocol, please refer to Moosmann et al. (2022) and Wagner et al. (2022).

KW - CRISPR

KW - Cell Biology

KW - Cell culture

KW - Cell-based Assays

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SARS-CoV-2 CD8⁺ T cell killing assays using replicating viruses and transgenic antigens

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Keywords

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