Safeguarding our genetic resources with libraries of doubled-haploid lines

Albrecht E. Melchinger, Pascal Schopp, Dominik Müller, Tobias A. Schrag, Eva Bauer, Sandra Unterseer, Linda Homann, Wolfgang Schipprack, Chris Carolin Schön

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Thousands of landraces are stored in seed banks as “gold reserves” for future use in plant breeding. In many crops, their utilization is hampered because they represent heterogeneous populations of heterozygous genotypes, which harbor a high genetic load. We show, with high-density genotyping in five landraces of maize, that libraries of doubled-haploid (DH) lines capture the allelic diversity of genetic resources in an unbiased way. By comparing allelic differentiation between heterozygous plants from the original landraces and 266 derived DH lines, we find conclusive evidence that, in the DH production process, sampling of alleles is random across the entire allele frequency spectrum, and purging of landraces from their genetic load does not act on specific genomic regions. Based on overall process efficiency, we show that generating DH lines is feasible for genetic material that has never been selected for inbreeding tolerance. We conclude that libraries of DH lines will make genetic resources accessible to crop improvement by linking molecular inventories of seed banks with meaningful phenotypes.

Original languageEnglish
Pages (from-to)1611-1619
Number of pages9
JournalGenetics
Volume206
Issue number3
DOIs
StatePublished - Jul 2017

Keywords

  • Allelic diversity
  • Genetic load
  • Haploidy linkage disequilibrium
  • Maize

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