RNAi-induced silencing of gene expression in strawberry fruit (Fragaria x ananassa) by agroinfiltration: A rapid assay for gene function analysis

Thomas Hoffmann, Gregor Kalinowski, Wilfried Schwab

Research output: Contribution to journalArticlepeer-review

181 Scopus citations

Abstract

Intron-containing constructs encoding self-complementary 'hairpin' RNA (ihpRNA) have the potential to efficiently silence genes in a range of plant species. In this study we demonstrate the silencing of a ripening-related chalcone synthase (CHS) gene in strawberry fruits (Fragaria x ananassa cv. Elsanta) by a construct (ihpRNA) containing the partial sense and corresponding antisense sequences of CHS separated by an intron obtained from a F. x ananassa quinone oxidoreductase gene. An Agrobacterium strain carrying a T-DNA expressing the ihpRNA transgene was injected with a syringe into the receptacles of growing fruits still attached to the plant about 14 days after pollination. As a consequence of the reduced levels of CHS mRNA and enzymatic CHS activity, the levels of anthocyanins were downregulated and precursors of the flavonoid pathway were shunted to the phenylpropanoid pathway leading to a large increases in levels of (hydroxy) cinnamoyl glucose esters. We anticipate that this technique in combination with metabolite profiling analysis will be useful for studying the function of unknown genes during the development and ripening of strawberry fruit.

Original languageEnglish
Pages (from-to)818-826
Number of pages9
JournalPlant Journal
Volume48
Issue number5
DOIs
StatePublished - Dec 2006

Keywords

  • Chalcone synthase
  • Fragaria x ananassa
  • Phenylpropanoid
  • Self-complementary hairpin RNA
  • Strawberry

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