RNA interference in mice

R. Kühn, S. Streif, W. Wurst

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

25 Scopus citations

Abstract

Silencing of gene expression by RNA interference (RNAi) has become a powerful tool for functional genomics in mammalian cells. Furthermore, RNAi holds promise as a simple, fast and cost-effective approach to studying mammalian gene function in vivo and as a novel therapeutic approach. This review provides an overview of the progress of RNAi in vivo, with emphasis on systemic/local siRNA delivery, viral shRNA vectors, shRNA vector transgenic mice and conditional systems to control shRNA vectors. Taken together, the data from 80 in vivo studies show that RNAi is a useful tool that offers new opportunities for functional genomics in mice.

Original languageEnglish
Title of host publicationConditional Mutagenesis
Subtitle of host publicationAn Approach to Disease Models
PublisherSpringer Science and Business Media, LLC
Pages149-176
Number of pages28
ISBN (Print)9783540351085
DOIs
StatePublished - 2007
Externally publishedYes

Publication series

NameHandbook of Experimental Pharmacology
Volume178
ISSN (Print)0171-2004
ISSN (Electronic)1865-0325

Keywords

  • Functional genomics
  • Mouse
  • RNAi
  • Transgenic mice
  • siRNA

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