RIP3 and AtKinesin-13A - A novel interaction linking Rho proteins of plants to microtubules

Elena Mucha; Caroline Hoefle; Ralph Hückelhoven; Antje Berken

doi:10.1016/j.ejcb.2010.08.003

RIP3 and AtKinesin-13A - A novel interaction linking Rho proteins of plants to microtubules

Elena Mucha, Caroline Hoefle, Ralph Hückelhoven, Antje Berken

Chair of Phytopathology

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39 Scopus citations

Abstract

RIP3 belongs to a group of recently identified proteins, classified as the ICR/RIP family whose members were described to interact with Rho proteins of plants (ROPs). Our in vivo and in vitro data demonstrate that RIP3 is a true ROP effector, interacting specifically with the active form of ROPs. We found that RIP3 has properties and cellular roles different from the previously described RIP family member ICR1/RIP1. We show that RIP3 is localized at microtubules and interacts with the kinesin-13 family member AtKinesin-13A, suggesting a role for RIP3 in microtubule reorganization and a possible function in ROP-regulated polar growth.

Original language	English
Pages (from-to)	906-916
Number of pages	11
Journal	European Journal of Cell Biology
Volume	89
Issue number	12
DOIs	https://doi.org/10.1016/j.ejcb.2010.08.003
State	Published - Dec 2010

Keywords

AtKinesin-13A
Cytoskeleton
Effector
ICR
Microtubule
Plant
Polar growth
RIP
ROP
Rho

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10.1016/j.ejcb.2010.08.003

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@article{a1534f37109d48f5b1174669557ef1e0,

title = "RIP3 and AtKinesin-13A - A novel interaction linking Rho proteins of plants to microtubules",

abstract = "RIP3 belongs to a group of recently identified proteins, classified as the ICR/RIP family whose members were described to interact with Rho proteins of plants (ROPs). Our in vivo and in vitro data demonstrate that RIP3 is a true ROP effector, interacting specifically with the active form of ROPs. We found that RIP3 has properties and cellular roles different from the previously described RIP family member ICR1/RIP1. We show that RIP3 is localized at microtubules and interacts with the kinesin-13 family member AtKinesin-13A, suggesting a role for RIP3 in microtubule reorganization and a possible function in ROP-regulated polar growth.",

keywords = "AtKinesin-13A, Cytoskeleton, Effector, ICR, Microtubule, Plant, Polar growth, RIP, ROP, Rho",

author = "Elena Mucha and Caroline Hoefle and Ralph H{\"u}ckelhoven and Antje Berken",

note = "Funding Information: We are grateful to Michal H{\'a}la and Viktor {\v Z}{\'a}rsk{\'y} who provided the exocyst clones. We thank Stephan Pollmann for plant expression vectors and Beate Voss for technical assistance. We thank the Deutsche Forschungsgemeinschaft for financial support (SFB480 project A11, SPP1150).",

year = "2010",

month = dec,

doi = "10.1016/j.ejcb.2010.08.003",

language = "English",

volume = "89",

pages = "906--916",

journal = "European Journal of Cell Biology",

issn = "0171-9335",

publisher = "Elsevier GmbH",

number = "12",

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TY - JOUR

T1 - RIP3 and AtKinesin-13A - A novel interaction linking Rho proteins of plants to microtubules

AU - Mucha, Elena

AU - Hoefle, Caroline

AU - Hückelhoven, Ralph

AU - Berken, Antje

N1 - Funding Information: We are grateful to Michal Hála and Viktor Žárský who provided the exocyst clones. We thank Stephan Pollmann for plant expression vectors and Beate Voss for technical assistance. We thank the Deutsche Forschungsgemeinschaft for financial support (SFB480 project A11, SPP1150).

PY - 2010/12

Y1 - 2010/12

N2 - RIP3 belongs to a group of recently identified proteins, classified as the ICR/RIP family whose members were described to interact with Rho proteins of plants (ROPs). Our in vivo and in vitro data demonstrate that RIP3 is a true ROP effector, interacting specifically with the active form of ROPs. We found that RIP3 has properties and cellular roles different from the previously described RIP family member ICR1/RIP1. We show that RIP3 is localized at microtubules and interacts with the kinesin-13 family member AtKinesin-13A, suggesting a role for RIP3 in microtubule reorganization and a possible function in ROP-regulated polar growth.

AB - RIP3 belongs to a group of recently identified proteins, classified as the ICR/RIP family whose members were described to interact with Rho proteins of plants (ROPs). Our in vivo and in vitro data demonstrate that RIP3 is a true ROP effector, interacting specifically with the active form of ROPs. We found that RIP3 has properties and cellular roles different from the previously described RIP family member ICR1/RIP1. We show that RIP3 is localized at microtubules and interacts with the kinesin-13 family member AtKinesin-13A, suggesting a role for RIP3 in microtubule reorganization and a possible function in ROP-regulated polar growth.

KW - AtKinesin-13A

KW - Cytoskeleton

KW - Effector

KW - ICR

KW - Microtubule

KW - Plant

KW - Polar growth

KW - RIP

KW - ROP

KW - Rho

UR - http://www.scopus.com/inward/record.url?scp=78149411424&partnerID=8YFLogxK

U2 - 10.1016/j.ejcb.2010.08.003

DO - 10.1016/j.ejcb.2010.08.003

M3 - Article

C2 - 20832900

AN - SCOPUS:78149411424

SN - 0171-9335

VL - 89

SP - 906

EP - 916

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

IS - 12

ER -

RIP3 and AtKinesin-13A - A novel interaction linking Rho proteins of plants to microtubules

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Keywords

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