Rezeptor-mediierter Gentransfer in Plattenepithelkarzinome des Kopf-Hals-Bereiches

B. Wollenberg; W. Schmidt; B. Schmitt; S. Lang; R. Zeidler

doi:10.1055/s-2007-997453

Rezeptor-mediierter Gentransfer in Plattenepithelkarzinome des Kopf-Hals-Bereiches

Translated title of the contribution: Receptor-mediated gene transfer into head and neck squamous cell cancer

B. Wollenberg, W. Schmidt, B. Schmitt, S. Lang, R. Zeidler

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The 'Adeno-Virus-Enhanced-Receptor-Mediated' (AVET)-System uses the receptor-mediated endocytosis route to introduce DNA into mammalian cells. By the help of a replication defective adenovirus with endosomolytic activity the coentry of receptor-bound DNA particles is faciliated. The transport to the nucleus followed by gene expression is multifold enhanced. Methods: Two different permanent cell lines from head and neck squamous cell cancer were transfected with the reporter gene for luciferase with either AVET or different liposoms in vitro. Results: In comparison it becomes obvious, that AVET shows a multifold higher transfection rate in vitro than the liposomal formulations applied. Conclusions: AVET could be used for efficient transfection of epithelial cells, that are fairly reluctant to DNA transfer. Further trials in the mouse model must prove a possible application in vivo.

Translated title of the contribution	Receptor-mediated gene transfer into head and neck squamous cell cancer
Original language	German
Pages (from-to)	411-414
Number of pages	4
Journal	Laryngo- Rhino- Otologie
Volume	76
Issue number	7
DOIs	https://doi.org/10.1055/s-2007-997453
State	Published - Jul 1997
Externally published	Yes

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title = "Rezeptor-mediierter Gentransfer in Plattenepithelkarzinome des Kopf-Hals-Bereiches",

abstract = "The 'Adeno-Virus-Enhanced-Receptor-Mediated' (AVET)-System uses the receptor-mediated endocytosis route to introduce DNA into mammalian cells. By the help of a replication defective adenovirus with endosomolytic activity the coentry of receptor-bound DNA particles is faciliated. The transport to the nucleus followed by gene expression is multifold enhanced. Methods: Two different permanent cell lines from head and neck squamous cell cancer were transfected with the reporter gene for luciferase with either AVET or different liposoms in vitro. Results: In comparison it becomes obvious, that AVET shows a multifold higher transfection rate in vitro than the liposomal formulations applied. Conclusions: AVET could be used for efficient transfection of epithelial cells, that are fairly reluctant to DNA transfer. Further trials in the mouse model must prove a possible application in vivo.",

keywords = "AVET, adenovirus, gene therapy, liposom, receptor-mediated gene transfer, squamous cell cancer",

author = "B. Wollenberg and W. Schmidt and B. Schmitt and S. Lang and R. Zeidler",

year = "1997",

month = jul,

doi = "10.1055/s-2007-997453",

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T1 - Rezeptor-mediierter Gentransfer in Plattenepithelkarzinome des Kopf-Hals-Bereiches

AU - Wollenberg, B.

AU - Schmidt, W.

AU - Schmitt, B.

AU - Lang, S.

AU - Zeidler, R.

PY - 1997/7

Y1 - 1997/7

N2 - The 'Adeno-Virus-Enhanced-Receptor-Mediated' (AVET)-System uses the receptor-mediated endocytosis route to introduce DNA into mammalian cells. By the help of a replication defective adenovirus with endosomolytic activity the coentry of receptor-bound DNA particles is faciliated. The transport to the nucleus followed by gene expression is multifold enhanced. Methods: Two different permanent cell lines from head and neck squamous cell cancer were transfected with the reporter gene for luciferase with either AVET or different liposoms in vitro. Results: In comparison it becomes obvious, that AVET shows a multifold higher transfection rate in vitro than the liposomal formulations applied. Conclusions: AVET could be used for efficient transfection of epithelial cells, that are fairly reluctant to DNA transfer. Further trials in the mouse model must prove a possible application in vivo.

AB - The 'Adeno-Virus-Enhanced-Receptor-Mediated' (AVET)-System uses the receptor-mediated endocytosis route to introduce DNA into mammalian cells. By the help of a replication defective adenovirus with endosomolytic activity the coentry of receptor-bound DNA particles is faciliated. The transport to the nucleus followed by gene expression is multifold enhanced. Methods: Two different permanent cell lines from head and neck squamous cell cancer were transfected with the reporter gene for luciferase with either AVET or different liposoms in vitro. Results: In comparison it becomes obvious, that AVET shows a multifold higher transfection rate in vitro than the liposomal formulations applied. Conclusions: AVET could be used for efficient transfection of epithelial cells, that are fairly reluctant to DNA transfer. Further trials in the mouse model must prove a possible application in vivo.

KW - AVET

KW - adenovirus

KW - gene therapy

KW - liposom

KW - receptor-mediated gene transfer

KW - squamous cell cancer

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ER -

Rezeptor-mediierter Gentransfer in Plattenepithelkarzinome des Kopf-Hals-Bereiches

Abstract

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