Abstract
trans-Resveratrol is a polyphenol found in blueberries, grapes, and wine with cancer chemopreventive properties. The low bioavailability of this compound enhances its concentration in the luminal content and becomes a potential chemopreventive agent against colon cancer. In the present study, the antiproliferative and pro-apoptotic effects on the human colorectal carcinoma HT-29 cells as well as the mechanisms underlying these effects were examined. Proliferation, cytotoxicity, and apoptosis were measured by fluorescence-based techniques. Studies of dose-dependent effects of trans-resveratrol showed antiproliferative activity with an EC50 value of 78.9 ± 5.4 μM. Caspase-3 was activated in a dose-dependent manner after incubation for 24 h giving an EC50 value of 276.1 ± 1.7 μM. Apoptosis was also confirmed with microscopic observation of changes in membrane permeability and detection of DNA fragmentation. The activity of trans-resveratrol on the mitochondria apoptosis pathway was evidenced by the production of superoxide anions in the mitochondria of cells undergoing apoptosis. In conclusion, trans-resveratrol inhibits cell proliferation without cytotoxicity and induces apoptosis in HT-29. Results of the present study provide evidence demonstrating the antitumor effect of trans-resveratrol via a ROS-dependent apoptosis pathway in colorectal carcinoma.
Original language | English |
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Pages (from-to) | 4813-4818 |
Number of pages | 6 |
Journal | Journal of agricultural and food chemistry |
Volume | 56 |
Issue number | 12 |
DOIs | |
State | Published - 25 Jun 2008 |
Keywords
- Apoptosis
- Caspase-3
- Colon cancer
- Proliferation
- ROS
- Resveratrol