Resveratrol induces apoptosis through ROS-dependent mitochondria pathway in HT-29 human colorectal carcinoma cells

M. Emília Juan, Uwe Wenzel, Hannelore Daniel, Joana M. Planas

Research output: Contribution to journalArticlepeer-review

179 Scopus citations

Abstract

trans-Resveratrol is a polyphenol found in blueberries, grapes, and wine with cancer chemopreventive properties. The low bioavailability of this compound enhances its concentration in the luminal content and becomes a potential chemopreventive agent against colon cancer. In the present study, the antiproliferative and pro-apoptotic effects on the human colorectal carcinoma HT-29 cells as well as the mechanisms underlying these effects were examined. Proliferation, cytotoxicity, and apoptosis were measured by fluorescence-based techniques. Studies of dose-dependent effects of trans-resveratrol showed antiproliferative activity with an EC50 value of 78.9 ± 5.4 μM. Caspase-3 was activated in a dose-dependent manner after incubation for 24 h giving an EC50 value of 276.1 ± 1.7 μM. Apoptosis was also confirmed with microscopic observation of changes in membrane permeability and detection of DNA fragmentation. The activity of trans-resveratrol on the mitochondria apoptosis pathway was evidenced by the production of superoxide anions in the mitochondria of cells undergoing apoptosis. In conclusion, trans-resveratrol inhibits cell proliferation without cytotoxicity and induces apoptosis in HT-29. Results of the present study provide evidence demonstrating the antitumor effect of trans-resveratrol via a ROS-dependent apoptosis pathway in colorectal carcinoma.

Original languageEnglish
Pages (from-to)4813-4818
Number of pages6
JournalJournal of agricultural and food chemistry
Volume56
Issue number12
DOIs
StatePublished - 25 Jun 2008

Keywords

  • Apoptosis
  • Caspase-3
  • Colon cancer
  • Proliferation
  • ROS
  • Resveratrol

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