Resolution enhancement in in vivo NMR spectroscopy: Detection of intermolecular zero-quantum coherences

Cornelius Faber, Eberhard Pracht, Axel Haase

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Intermolecular zero-quantum coherences are insensitive to magnetic field inhomogeneities. For this reason we have applied the HOMOGENIZED sequence [Vathyam et al., Science 272 (1996) 92] to phantoms containing metabolites at low concentrations, phantoms with air inclusions, an intact grape, and the head of a rat in vivo at 750MHz. In the 1H-spectra, the water signal is efficiently suppressed and line broadening due to susceptibility gradients is effectively removed along the indirectly detected dimension. We have obtained a 1H-spectrum of a 2.5mM solution of γ-aminobutyric acid in 12min scan time. In the phantom with air inclusions a reduction of line widths from 0.48ppm in the direct dimension to 0.07ppm in the indirect dimension was observed, while in a deshimmed grape the reduction was from 1.4 to 0.07ppm. In a spectrum of the grape we were able to resolve glucose resonances at 0.3ppm from the water in 6min scan time. J-coupling information was partly retained. In the in vivo spectra of the rat brain five major metabolites were observed.

Original languageEnglish
Pages (from-to)265-274
Number of pages10
JournalJournal of Magnetic Resonance
Volume161
Issue number2
DOIs
StatePublished - Apr 2003
Externally publishedYes

Keywords

  • Dipolar demagnetizing field
  • HOMOGENIZED
  • In vivo NMR spectroscopy
  • Resolution enhancement
  • iZQC

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