TY - JOUR
T1 - Residual human immunodeficiency virus (HIV) type 1 RNA and DNA in lymph nodes and HIV RNA in genital secretions and in cerebrospinal fluid after suppression of viremia for 2 years
AU - Günthard, Huldrych F.
AU - Havlir, Diane V.
AU - Fiscus, Susan
AU - Zhang, Zhi Qiang
AU - Eron, Joseph
AU - Mellors, John
AU - Gulick, Roy
AU - Frost, Simon D.W.
AU - Brown, Andrew J.Leigh
AU - Schleif, William
AU - Valentine, Fred
AU - Jonas, Leslie
AU - Meibohm, Anne
AU - Ignacio, Caroline C.
AU - Isaacs, Robin
AU - Gamagami, Reza
AU - Emini, Emilio
AU - Haase, Ashley
AU - Richman, Douglas D.
AU - Wong, Joseph K.
N1 - Funding Information:
Financial support: National lnstitutes of Health (grants AI-43752 to J.K.W.; AI-27670, AI-38858, AI-29164, and AI-36214 to D.D.R.; HD-37260; and RR-00046); Swiss National Science Foundation (grant 84AD-046176 to H.F.G.); Medical Research Council Research fellowship (G81/ 298 to S.D.W.F.); Fogarty Foundation (to A.J.L.B.); Merck Research Laboratories (unrestricted educational grant); Research Center for AIDS and HIV Infection of the San Diego Veterans Affairs Healthcare System; VA Career Development Award (to J.K.W.).
PY - 2001/5/1
Y1 - 2001/5/1
N2 - Residual viral replication persists in a significant proportion of human immunodeficiency virus (HIV)-infected patients receiving potent antiretroviral therapy. To determine the source of this virus, levels of HIV RNA and DNA from lymphoid tissues and levels of viral RNA in serum, cerebrospinal fluid (CSF), and genital secretions in 28 patients treated for ≤2.5 years with indinavir, zidovudine, and lamivudine were examined. Both HIV RNA and DNA remained detectable in all lymph nodes. In contrast, HIV RNA was not detected in 20 of 23 genital secretions or in any of 13 CSF samples after 2 years of treatment. HIV envelope sequence data from plasma and lymph nodes from 4 patients demonstrated sequence divergence, which suggests varying degrees of residual viral replication in 3 and absence in 1 patient. In patients receiving potent antiretroviral therapy, the greatest virus burden may continue to be in lymphoid tissues rather than in central nervous system or genitourinary compartments.
AB - Residual viral replication persists in a significant proportion of human immunodeficiency virus (HIV)-infected patients receiving potent antiretroviral therapy. To determine the source of this virus, levels of HIV RNA and DNA from lymphoid tissues and levels of viral RNA in serum, cerebrospinal fluid (CSF), and genital secretions in 28 patients treated for ≤2.5 years with indinavir, zidovudine, and lamivudine were examined. Both HIV RNA and DNA remained detectable in all lymph nodes. In contrast, HIV RNA was not detected in 20 of 23 genital secretions or in any of 13 CSF samples after 2 years of treatment. HIV envelope sequence data from plasma and lymph nodes from 4 patients demonstrated sequence divergence, which suggests varying degrees of residual viral replication in 3 and absence in 1 patient. In patients receiving potent antiretroviral therapy, the greatest virus burden may continue to be in lymphoid tissues rather than in central nervous system or genitourinary compartments.
UR - http://www.scopus.com/inward/record.url?scp=0035341524&partnerID=8YFLogxK
U2 - 10.1086/319864
DO - 10.1086/319864
M3 - Article
C2 - 11294662
AN - SCOPUS:0035341524
SN - 0022-1899
VL - 183
SP - 1318
EP - 1327
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 9
ER -
