TY - JOUR
T1 - Replication of the broad host range plasmid RSF1010 in cell-free extracts of Escherichia coli and Pseudomonas aeruginosa
AU - Diaz, Ramon
AU - Staudenbauer, Walter L.
PY - 1982/8/11
Y1 - 1982/8/11
N2 - Replication of exogenous RSF1010 DNA can be carried out by soluble enzyme systems from Escherichia coli and Pseudomonas aeruginosa. It requires the function of RSF1010-encoded replication protein(s), which is not expressed in extracts from plasmid-free bacteria. In contrast to previously described in vitro systems for plasmid replication, initiation of RSF1010 DNA synthesis is independent of transcription catalyzed by host RNA polymerase. This is indicated by the insensitivity of RSF1010 replication to rifampicin as well as to RNA polymerase antibodies. It is proposed that a host RNA polymerase transcription-independent initiation mechanism might be a general property of broad host range plasmids.
AB - Replication of exogenous RSF1010 DNA can be carried out by soluble enzyme systems from Escherichia coli and Pseudomonas aeruginosa. It requires the function of RSF1010-encoded replication protein(s), which is not expressed in extracts from plasmid-free bacteria. In contrast to previously described in vitro systems for plasmid replication, initiation of RSF1010 DNA synthesis is independent of transcription catalyzed by host RNA polymerase. This is indicated by the insensitivity of RSF1010 replication to rifampicin as well as to RNA polymerase antibodies. It is proposed that a host RNA polymerase transcription-independent initiation mechanism might be a general property of broad host range plasmids.
UR - http://www.scopus.com/inward/record.url?scp=0020480432&partnerID=8YFLogxK
U2 - 10.1093/nar/10.15.4687
DO - 10.1093/nar/10.15.4687
M3 - Article
C2 - 6813825
AN - SCOPUS:0020480432
SN - 0305-1048
VL - 10
SP - 4687
EP - 4702
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 15
ER -