Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood

Tino F. Schwarz; Stefan Serke; Barbara Hottenträger; Albrecht Von Brunn; Herrad Baurmann; Andreas Kirsch; Wilhelm Stolz; Dieter Huhn; Friedrich Deinhardt; Michael Roggendorf

Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood

Tino F. Schwarz
, Stefan Serke
, Barbara Hottenträger
, Albrecht Von Brunn
, Herrad Baurmann
, Andreas Kirsch
, Wilhelm Stolz
, Dieter Huhn
, Friedrich Deinhardt
, Michael Roggendorf

University of Munich
Charité – Universitätsmedizin Berlin
Free University of Berlin

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.

Original language	English
Pages (from-to)	1273-1276
Number of pages	4
Journal	Journal of Virology
Volume	66
Issue number	2
State	Published - Feb 1992
Externally published	Yes

Cite this

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title = "Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood",

abstract = "Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.",

author = "Schwarz, \{Tino F.\} and Stefan Serke and Barbara Hottentr{\"a}ger and \{Von Brunn\}, Albrecht and Herrad Baurmann and Andreas Kirsch and Wilhelm Stolz and Dieter Huhn and Friedrich Deinhardt and Michael Roggendorf",

year = "1992",

month = feb,

language = "English",

volume = "66",

pages = "1273--1276",

journal = "Journal of Virology",

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T1 - Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood

AU - Schwarz, Tino F.

AU - Serke, Stefan

AU - Hottenträger, Barbara

AU - Von Brunn, Albrecht

AU - Baurmann, Herrad

AU - Kirsch, Andreas

AU - Stolz, Wilhelm

AU - Huhn, Dieter

AU - Deinhardt, Friedrich

AU - Roggendorf, Michael

PY - 1992/2

Y1 - 1992/2

N2 - Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.

AB - Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.

UR - https://www.scopus.com/pages/publications/0026543322

M3 - Article

C2 - 1731104

AN - SCOPUS:0026543322

SN - 0022-538X

VL - 66

SP - 1273

EP - 1276

JO - Journal of Virology

JF - Journal of Virology

IS - 2

ER -

Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood

Abstract

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