Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood

Tino F. Schwarz; Stefan Serke; Barbara Hottenträger; Albrecht Von Brunn; Herrad Baurmann; Andreas Kirsch; Wilhelm Stolz; Dieter Huhn; Friedrich Deinhardt; Michael Roggendorf

Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood

Tino F. Schwarz, Stefan Serke, Barbara Hottenträger, Albrecht Von Brunn, Herrad Baurmann, Andreas Kirsch, Wilhelm Stolz, Dieter Huhn, Friedrich Deinhardt, Michael Roggendorf

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.

Original language	English
Pages (from-to)	1273-1276
Number of pages	4
Journal	Journal of Virology
Volume	66
Issue number	2
State	Published - Feb 1992
Externally published	Yes

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title = "Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood",

abstract = "Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.",

author = "Schwarz, {Tino F.} and Stefan Serke and Barbara Hottentr{\"a}ger and {Von Brunn}, Albrecht and Herrad Baurmann and Andreas Kirsch and Wilhelm Stolz and Dieter Huhn and Friedrich Deinhardt and Michael Roggendorf",

year = "1992",

month = feb,

language = "English",

volume = "66",

pages = "1273--1276",

journal = "Journal of Virology",

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T1 - Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood

AU - Schwarz, Tino F.

AU - Serke, Stefan

AU - Hottenträger, Barbara

AU - Von Brunn, Albrecht

AU - Baurmann, Herrad

AU - Kirsch, Andreas

AU - Stolz, Wilhelm

AU - Huhn, Dieter

AU - Deinhardt, Friedrich

AU - Roggendorf, Michael

PY - 1992/2

Y1 - 1992/2

N2 - Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.

AB - Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.

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M3 - Article

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AN - SCOPUS:0026543322

SN - 0022-538X

VL - 66

SP - 1273

EP - 1276

JO - Journal of Virology

JF - Journal of Virology

IS - 2

ER -

Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood

Abstract

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