Replication of Colicinogenic Factor E1 DNA in Plasmolysed Escherichia coli Cells: Coupling of DNA Replication and RNA Synthesis

Plasmolysed chloramphenicol‐treated Escherichia coli cells carrying the colicinogenic factor E1 utilize deoxynucleoside triphosphates for the semi‐conservative synthesis of Col E1 DNA. Col E1 DNA replication in plasmolysed cells can be dissociated into two temporally separated processes: (a) a rifampicin‐sensitive RNA synthesis, which is stimulated by adenosine 3′:5′‐monophosphate (cyclic AMP) and requires all four ribonucleoside triphosphates and (b) an ATP‐dependent DNA synthesis, which is inhibited by arabinosylnucleoside triphosphates and sulfhydryl‐blocking reagents. These two processes exhibit different sensitivities to inhibition by polyamines and actinomycin D.