Replication of colicinogenic factor E 1 DNA: Evidence for a discontinuous replication mechanism

Walter L. Staudenbauer

doi:10.1093/nar/1.9.1153

Replication of colicinogenic factor E 1 DNA: Evidence for a discontinuous replication mechanism

Walter L. Staudenbauer

Max Planck Institute of Biochemistry

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The mechanism of Col E 1 DNA replication was investigated in a plasmolysed cell system prepared from chloramphenicol-treated E. coli JC 411 (Col E 1). After pulse-labelling with ³H-dTTP a considerable fraction of the newly synthesized DNA was recovered as single-stranded fragments. Upon alkali denaturation the pulse label was found in DNA chains sedimenting slower than unit length Col E 1 strands with a prominent peak at 5 S. During a chase with unlabeled precursors the label is transferred nearly completely into supercoiled Col E 1 DNA. DNA ligase appears to be required for the joining of the 5 Spieces since in the absence of NAD an accumulation of short fragments is observed.

Original language	English
Pages (from-to)	1153-1164
Number of pages	12
Journal	Nucleic Acids Research
Volume	1
Issue number	9
DOIs	https://doi.org/10.1093/nar/1.9.1153
State	Published - Sep 1974
Externally published	Yes

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title = "Replication of colicinogenic factor E 1 DNA: Evidence for a discontinuous replication mechanism",

abstract = "The mechanism of Col E 1 DNA replication was investigated in a plasmolysed cell system prepared from chloramphenicol-treated E. coli JC 411 (Col E 1). After pulse-labelling with 3H-dTTP a considerable fraction of the newly synthesized DNA was recovered as single-stranded fragments. Upon alkali denaturation the pulse label was found in DNA chains sedimenting slower than unit length Col E 1 strands with a prominent peak at 5 S. During a chase with unlabeled precursors the label is transferred nearly completely into supercoiled Col E 1 DNA. DNA ligase appears to be required for the joining of the 5 Spieces since in the absence of NAD an accumulation of short fragments is observed.",

author = "Staudenbauer, \{Walter L.\}",

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N2 - The mechanism of Col E 1 DNA replication was investigated in a plasmolysed cell system prepared from chloramphenicol-treated E. coli JC 411 (Col E 1). After pulse-labelling with 3H-dTTP a considerable fraction of the newly synthesized DNA was recovered as single-stranded fragments. Upon alkali denaturation the pulse label was found in DNA chains sedimenting slower than unit length Col E 1 strands with a prominent peak at 5 S. During a chase with unlabeled precursors the label is transferred nearly completely into supercoiled Col E 1 DNA. DNA ligase appears to be required for the joining of the 5 Spieces since in the absence of NAD an accumulation of short fragments is observed.

AB - The mechanism of Col E 1 DNA replication was investigated in a plasmolysed cell system prepared from chloramphenicol-treated E. coli JC 411 (Col E 1). After pulse-labelling with 3H-dTTP a considerable fraction of the newly synthesized DNA was recovered as single-stranded fragments. Upon alkali denaturation the pulse label was found in DNA chains sedimenting slower than unit length Col E 1 strands with a prominent peak at 5 S. During a chase with unlabeled precursors the label is transferred nearly completely into supercoiled Col E 1 DNA. DNA ligase appears to be required for the joining of the 5 Spieces since in the absence of NAD an accumulation of short fragments is observed.

UR - https://www.scopus.com/pages/publications/0016098149

U2 - 10.1093/nar/1.9.1153

DO - 10.1093/nar/1.9.1153

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EP - 1164

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 9

ER -

Replication of colicinogenic factor E 1 DNA: Evidence for a discontinuous replication mechanism

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