TY - JOUR
T1 - Replication of colicinogenic factor E 1 DNA
T2 - Evidence for a discontinuous replication mechanism
AU - Staudenbauer, Walter L.
PY - 1974/9
Y1 - 1974/9
N2 - The mechanism of Col E 1 DNA replication was investigated in a plasmolysed cell system prepared from chloramphenicol-treated E. coli JC 411 (Col E 1). After pulse-labelling with 3H-dTTP a considerable fraction of the newly synthesized DNA was recovered as single-stranded fragments. Upon alkali denaturation the pulse label was found in DNA chains sedimenting slower than unit length Col E 1 strands with a prominent peak at 5 S. During a chase with unlabeled precursors the label is transferred nearly completely into supercoiled Col E 1 DNA. DNA ligase appears to be required for the joining of the 5 Spieces since in the absence of NAD an accumulation of short fragments is observed.
AB - The mechanism of Col E 1 DNA replication was investigated in a plasmolysed cell system prepared from chloramphenicol-treated E. coli JC 411 (Col E 1). After pulse-labelling with 3H-dTTP a considerable fraction of the newly synthesized DNA was recovered as single-stranded fragments. Upon alkali denaturation the pulse label was found in DNA chains sedimenting slower than unit length Col E 1 strands with a prominent peak at 5 S. During a chase with unlabeled precursors the label is transferred nearly completely into supercoiled Col E 1 DNA. DNA ligase appears to be required for the joining of the 5 Spieces since in the absence of NAD an accumulation of short fragments is observed.
UR - http://www.scopus.com/inward/record.url?scp=0016098149&partnerID=8YFLogxK
U2 - 10.1093/nar/1.9.1153
DO - 10.1093/nar/1.9.1153
M3 - Article
C2 - 4616225
AN - SCOPUS:0016098149
SN - 0305-1048
VL - 1
SP - 1153
EP - 1164
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 9
ER -