Replication of Bacteriophage M‐13: Template Specific Inhibition of DNA Synthesis by Nalidixic Acid

Peter K. Schneck, Walter L. Staudenbauer, Peter Hans Hofschneider

Research output: Contribution to journalArticlepeer-review

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Abstract

Nalidixic acid, a specific inhibitor of bacterial DNA synthesis, has no effect on the conversion of M‐13 parental or progeny single strands into double‐stranded replicative form molecules. However, the double strand to double strand replication of the replicative forms as well as the synthesis of single‐stranded M‐13 DNA are completely inhibited. As in the case of M‐13, the formation of the parental replicative form of ΨX 174 is also insensitive to nalidixic acid. It is concluded that nalidixic acid (or some metabolic product thereof) interferes with DNA replication only if a double‐stranded template is involved, but has no effect on the synthesis of a complementary strand on various single‐stranded templates.

Original languageEnglish
Pages (from-to)130-136
Number of pages7
JournalEuropean Journal of Biochemistry
Volume38
Issue number1
DOIs
StatePublished - Sep 1973
Externally publishedYes

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