Removal of monomer delignification products by laccase from Trametes versicolor

Michaela Kolb; Volker Sieber; Manfred Amann; Martin Faulstich; Doris Schieder

doi:10.1016/j.biortech.2011.11.080

Removal of monomer delignification products by laccase from Trametes versicolor

Michaela Kolb, Volker Sieber, Manfred Amann, Martin Faulstich, Doris Schieder

Chair of Chemistry of Biogenic Resources

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

The influence of a laccase from Trametes versicolor on the removal of phenolic monomers in liquid hot water pretreated wheat straw supernatants (LHW-S) was examined. Beside the total phenol content derived by Folin-Ciocalteu (FC-) assay, phenolic monomers were measured via headspace-solid phase micro-extraction (HS-SPME)/GC-MS. A notable decrease of the phenols was achieved using 0.2 and 0.5. U/mL laccase whilst higher dosage showed no improvement. Nearly all kind of monomer phenolic compounds identified in the LHW-S were found to be removed after 24. h. However, acetophenone and 4-hydroxybenzaldehyde (HBA) were obviously not affected by laccase. Summarizing, three laccase reaction groups (LRG) of phenolic monomers could be classified: immediate removal (LRG-A), degradation after 1. day (LRG-B), no effect of laccase (LRG-C). Additionally, HS-SPME/GC was found to be a powerful tool to study the reaction of laccase and phenolic monomers in complex lignocellulose derived solutions.

Original language	English
Pages (from-to)	298-304
Number of pages	7
Journal	Bioresource Technology
Volume	104
DOIs	https://doi.org/10.1016/j.biortech.2011.11.080
State	Published - Jan 2012

Keywords

Detoxification
Folin-Ciocalteu assay
Headspace-SPME/GC-MS
Laccase
Lignocellulose

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2011.11.080

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title = "Removal of monomer delignification products by laccase from Trametes versicolor",

abstract = "The influence of a laccase from Trametes versicolor on the removal of phenolic monomers in liquid hot water pretreated wheat straw supernatants (LHW-S) was examined. Beside the total phenol content derived by Folin-Ciocalteu (FC-) assay, phenolic monomers were measured via headspace-solid phase micro-extraction (HS-SPME)/GC-MS. A notable decrease of the phenols was achieved using 0.2 and 0.5. U/mL laccase whilst higher dosage showed no improvement. Nearly all kind of monomer phenolic compounds identified in the LHW-S were found to be removed after 24. h. However, acetophenone and 4-hydroxybenzaldehyde (HBA) were obviously not affected by laccase. Summarizing, three laccase reaction groups (LRG) of phenolic monomers could be classified: immediate removal (LRG-A), degradation after 1. day (LRG-B), no effect of laccase (LRG-C). Additionally, HS-SPME/GC was found to be a powerful tool to study the reaction of laccase and phenolic monomers in complex lignocellulose derived solutions.",

keywords = "Detoxification, Folin-Ciocalteu assay, Headspace-SPME/GC-MS, Laccase, Lignocellulose",

author = "Michaela Kolb and Volker Sieber and Manfred Amann and Martin Faulstich and Doris Schieder",

note = "Funding Information: The authors gratefully acknowledge the funding received from Wacker Chemie AG and the Federal Ministry of Education and Research, Germany ( FKZ: 0315193 ). Furthermore, acknowledgements are expressed to the Deutsche Forschungsgemeinschaft DFG for financial support of the Tecan workstation (DFG-GZ: INST 95/1061-1 FUGG ).",

year = "2012",

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doi = "10.1016/j.biortech.2011.11.080",

language = "English",

volume = "104",

pages = "298--304",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd.",

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T1 - Removal of monomer delignification products by laccase from Trametes versicolor

AU - Kolb, Michaela

AU - Sieber, Volker

AU - Amann, Manfred

AU - Faulstich, Martin

AU - Schieder, Doris

N1 - Funding Information: The authors gratefully acknowledge the funding received from Wacker Chemie AG and the Federal Ministry of Education and Research, Germany ( FKZ: 0315193 ). Furthermore, acknowledgements are expressed to the Deutsche Forschungsgemeinschaft DFG for financial support of the Tecan workstation (DFG-GZ: INST 95/1061-1 FUGG ).

PY - 2012/1

Y1 - 2012/1

N2 - The influence of a laccase from Trametes versicolor on the removal of phenolic monomers in liquid hot water pretreated wheat straw supernatants (LHW-S) was examined. Beside the total phenol content derived by Folin-Ciocalteu (FC-) assay, phenolic monomers were measured via headspace-solid phase micro-extraction (HS-SPME)/GC-MS. A notable decrease of the phenols was achieved using 0.2 and 0.5. U/mL laccase whilst higher dosage showed no improvement. Nearly all kind of monomer phenolic compounds identified in the LHW-S were found to be removed after 24. h. However, acetophenone and 4-hydroxybenzaldehyde (HBA) were obviously not affected by laccase. Summarizing, three laccase reaction groups (LRG) of phenolic monomers could be classified: immediate removal (LRG-A), degradation after 1. day (LRG-B), no effect of laccase (LRG-C). Additionally, HS-SPME/GC was found to be a powerful tool to study the reaction of laccase and phenolic monomers in complex lignocellulose derived solutions.

AB - The influence of a laccase from Trametes versicolor on the removal of phenolic monomers in liquid hot water pretreated wheat straw supernatants (LHW-S) was examined. Beside the total phenol content derived by Folin-Ciocalteu (FC-) assay, phenolic monomers were measured via headspace-solid phase micro-extraction (HS-SPME)/GC-MS. A notable decrease of the phenols was achieved using 0.2 and 0.5. U/mL laccase whilst higher dosage showed no improvement. Nearly all kind of monomer phenolic compounds identified in the LHW-S were found to be removed after 24. h. However, acetophenone and 4-hydroxybenzaldehyde (HBA) were obviously not affected by laccase. Summarizing, three laccase reaction groups (LRG) of phenolic monomers could be classified: immediate removal (LRG-A), degradation after 1. day (LRG-B), no effect of laccase (LRG-C). Additionally, HS-SPME/GC was found to be a powerful tool to study the reaction of laccase and phenolic monomers in complex lignocellulose derived solutions.

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KW - Folin-Ciocalteu assay

KW - Headspace-SPME/GC-MS

KW - Laccase

KW - Lignocellulose

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Removal of monomer delignification products by laccase from Trametes versicolor

Abstract

Keywords

UN SDGs

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