Relative orientation of rigid nitroxides by peldor: Beyond distance measurements in nucleic acids

Olav Schiemann, Pavol Cekan, Dominik Margraf, Thomas F. Prisner, Snorri Th Sigurdsson

Research output: Contribution to journalArticlepeer-review

170 Scopus citations

Abstract

Show me your angle: Incorporation of the rigid spin label Ç allows determination of both distance and orientation of two nitroxide spin labels in DNA by PELDOR experiments at common X-band frequencies. The orientational information is obtained by varying the position of the detection pulses over the nitroxide spectrum. Simulation of the set of time traces yields very precise distances and angles.

Original languageEnglish
Pages (from-to)3292-3295
Number of pages4
JournalAngewandte Chemie International Edition in English
Volume48
Issue number18
DOIs
StatePublished - 20 Apr 2009
Externally publishedYes

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