TY - JOUR
T1 - Regulatory variability of camalexin biosynthesis
AU - Schuhegger, Regina
AU - Rauhut, Thomas
AU - Glawischnig, Erich
N1 - Funding Information:
We kindly thank Prof. Alfons Gierl for his support and helpful suggestions, Prof. Barbara Halkier and Dr. Michael Mikkelsen for providing seeds of CYP79B2::GUS plants, Dr. Günther Bahnweg for his advice in Alternaria culture and providing the strain, and Dr. Monika Frey for critical reading of the manuscript. This work has been funded by the Deutsche Forschungsgemeinschaft (GL346/1).
PY - 2007/5/3
Y1 - 2007/5/3
N2 - The anthranilate synthase ASA1, CYP79B2 and CYP71B15 (PAD3) are biosynthetic genes of the Arabidopsis phytoalexin camalexin, which are induced after pathogen infection and abiotic treatments like silver nitrate spraying. The natural variation of camalexin biosynthesis in response to Pseudomonas syringae infection was determined in several ecotypes, and differential CYP71B15 regulation as a potential basis for this variation was investigated. The expression of camalexin biosynthetic genes was restricted to the tissue undergoing cell death. After droplet infection with Alternaria alternata, a potent camalexin inducer in the Col-0 ecotype, camalexin formation and the induction of ASA1, CYP79B2 and CYP71B15 were strictly co-localized with the infection site.
AB - The anthranilate synthase ASA1, CYP79B2 and CYP71B15 (PAD3) are biosynthetic genes of the Arabidopsis phytoalexin camalexin, which are induced after pathogen infection and abiotic treatments like silver nitrate spraying. The natural variation of camalexin biosynthesis in response to Pseudomonas syringae infection was determined in several ecotypes, and differential CYP71B15 regulation as a potential basis for this variation was investigated. The expression of camalexin biosynthetic genes was restricted to the tissue undergoing cell death. After droplet infection with Alternaria alternata, a potent camalexin inducer in the Col-0 ecotype, camalexin formation and the induction of ASA1, CYP79B2 and CYP71B15 were strictly co-localized with the infection site.
KW - Alternaria alternata
KW - Arabidopsis thaliana
KW - Camalexin
KW - Pseudomonas syringae
UR - http://www.scopus.com/inward/record.url?scp=34047095999&partnerID=8YFLogxK
U2 - 10.1016/j.jplph.2006.04.012
DO - 10.1016/j.jplph.2006.04.012
M3 - Article
C2 - 16769150
AN - SCOPUS:34047095999
SN - 0176-1617
VL - 164
SP - 636
EP - 644
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 5
ER -