TY - JOUR
T1 - Region and cell-type resolved quantitative proteomic map of the human heart
AU - Doll, Sophia
AU - Dreßen, Martina
AU - Geyer, Philipp E.
AU - Itzhak, Daniel N.
AU - Braun, Christian
AU - Doppler, Stefanie A.
AU - Meier, Florian
AU - Deutsch, Marcus Andre
AU - Lahm, Harald
AU - Lange, Rüdiger
AU - Krane, Markus
AU - Mann, Matthias
N1 - Publisher Copyright:
© 2017 The Author(s).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - The heart is a central human organ and its diseases are the leading cause of death worldwide, but an in-depth knowledge of the identity and quantity of its constituent proteins is still lacking. Here, we determine the healthy human heart proteome by measuring 16 anatomical regions and three major cardiac cell types by high-resolution mass spectrometry-based proteomics. From low microgram sample amounts, we quantify over 10,700 proteins in this high dynamic range tissue. We combine copy numbers per cell with protein organellar assignments to build a model of the heart proteome at the subcellular level. Analysis of cardiac fibroblasts identifies cellular receptors as potential cell surface markers. Application of our heart map to atrial fibrillation reveals individually distinct mitochondrial dysfunctions. The heart map is available at maxqb.biochem.mpg.de as a resource for future analyses of normal heart function and disease.
AB - The heart is a central human organ and its diseases are the leading cause of death worldwide, but an in-depth knowledge of the identity and quantity of its constituent proteins is still lacking. Here, we determine the healthy human heart proteome by measuring 16 anatomical regions and three major cardiac cell types by high-resolution mass spectrometry-based proteomics. From low microgram sample amounts, we quantify over 10,700 proteins in this high dynamic range tissue. We combine copy numbers per cell with protein organellar assignments to build a model of the heart proteome at the subcellular level. Analysis of cardiac fibroblasts identifies cellular receptors as potential cell surface markers. Application of our heart map to atrial fibrillation reveals individually distinct mitochondrial dysfunctions. The heart map is available at maxqb.biochem.mpg.de as a resource for future analyses of normal heart function and disease.
UR - http://www.scopus.com/inward/record.url?scp=85034242143&partnerID=8YFLogxK
U2 - 10.1038/s41467-017-01747-2
DO - 10.1038/s41467-017-01747-2
M3 - Article
C2 - 29133944
AN - SCOPUS:85034242143
SN - 2041-1723
VL - 8
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1469
ER -
