Regeneration of isolated protoplasts from nicotinic acid-deficient mutants of the liverwort Sphaerocarpos donnellii aust

Gerhard Wenzel, Otto Schieder, Gerhard Wenzel, Otto Schieder

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Abstract

Protoplasts from two different nicotinic acid-deficient strains of Sphaero-carpos were obtained by enzymatic degratation of cell walls. Regeneration of cell wall occured oon a culture medium based on natural seawater diluted to 60%. After stepwise transfer to Benecke-agar the newly formed cells developed to normal haploidsplants.

Original languageEnglish
Pages (from-to)421-423
Number of pages3
JournalPlant Science Letters
Volume1
Issue number11
DOIs
StatePublished - Nov 1973
Externally publishedYes

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