Regenerable immuno-biochip for screening ochratoxin A in green coffee extract using an automated microarray chip reader with chemiluminescence detection

Jimena C. Sauceda-Friebe, Xaver Y.Z. Karsunke, Susanna Vazac, Scarlett Biselli, Reinhard Niessner, Dietmar Knopp

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Ochratoxin A (OTA) can contaminate foodstuffs in the ppb to ppm range and once formed, it is difficult to remove. Because of its toxicity and potential risks to human health, the need exists for rapid, efficient detection methods that comply with legal maximum residual limits. In this work we have synthesized an OTA conjugate functionalized with a water-soluble peptide for covalent immobilization on a glass biochip by means of contact spotting. The chip was used for OTA determination with an indirect competitive immunoassay format with flow-through reagent addition and chemiluminescence detection, carried out with the stand-alone automated Munich Chip Reader 3 (MCR 3) platform. A buffer model and real green coffee extracts were used for this purpose. At the present, covalent conjugate immobilization allowed for at least 20 assay-regeneration cycles of the biochip surface. The total analysis time for a single sample, including measurement and surface regeneration, was 12min and the LOQ of OTA in green coffee extract was 0.3μgL-1 which corresponds to 7μgkg-1.

Original languageEnglish
Pages (from-to)234-242
Number of pages9
JournalAnalytica Chimica Acta
Volume689
Issue number2
DOIs
StatePublished - 18 Mar 2011

Keywords

  • Automated immunoassay
  • Chemiluminescence detection
  • Green coffee
  • Ochratoxin A (OTA)
  • Peptide-OTA conjugate
  • Regenerable biochip

Fingerprint

Dive into the research topics of 'Regenerable immuno-biochip for screening ochratoxin A in green coffee extract using an automated microarray chip reader with chemiluminescence detection'. Together they form a unique fingerprint.

Cite this