Recent Advances in the Monitoring of Protein Crystallization Processes in Downstream Processing

Brigitte Walla, Daniel Bischoff, Iliana Corona Viramontes, Sergio Montes Figueredo, Dirk Weuster-Botz

Research output: Contribution to journalReview articlepeer-review

2 Scopus citations

Abstract

Protein crystallization is nowadays performed at the micro to macro scale in academia and industry, being particularly interesting for pharmaceutical applications. Protein crystallization offers an attractive alternative to chromatography as a downstream processing step in the biotechnology industry, but also in the food and chemical industries. Monitoring of the protein crystallization process is required to understand the crystal growth mechanism and to obtain the information necessary for efficient process control, which needs to comply with the critical quality attributes of the product. Since a wide range of monitoring techniques have already been developed and established, this review focuses on the recent advances of selected techniques in monitoring protein crystallization processes such as the focused beam reflectance method (FBRM), and machine learning-based image analysis for solid-phase monitoring, as well as the spectroscopic methods for liquid-phase monitoring, such as attenuated total reflectance Fourier transform infrared (ATR-FTIR) and UV/Vis spectroscopy.

Original languageEnglish
Article number773
JournalCrystals
Volume13
Issue number5
DOIs
StatePublished - May 2023

Keywords

  • UV/Vis spectroscopy
  • attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy
  • focused beam reflectance measurement (FBRM)
  • machine learning (ML) based image analysis
  • monitoring techniques
  • process analytical technology (PAT)
  • protein crystallization

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