TY - JOUR
T1 - Recent Advances in the Monitoring of Protein Crystallization Processes in Downstream Processing
AU - Walla, Brigitte
AU - Bischoff, Daniel
AU - Corona Viramontes, Iliana
AU - Montes Figueredo, Sergio
AU - Weuster-Botz, Dirk
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/5
Y1 - 2023/5
N2 - Protein crystallization is nowadays performed at the micro to macro scale in academia and industry, being particularly interesting for pharmaceutical applications. Protein crystallization offers an attractive alternative to chromatography as a downstream processing step in the biotechnology industry, but also in the food and chemical industries. Monitoring of the protein crystallization process is required to understand the crystal growth mechanism and to obtain the information necessary for efficient process control, which needs to comply with the critical quality attributes of the product. Since a wide range of monitoring techniques have already been developed and established, this review focuses on the recent advances of selected techniques in monitoring protein crystallization processes such as the focused beam reflectance method (FBRM), and machine learning-based image analysis for solid-phase monitoring, as well as the spectroscopic methods for liquid-phase monitoring, such as attenuated total reflectance Fourier transform infrared (ATR-FTIR) and UV/Vis spectroscopy.
AB - Protein crystallization is nowadays performed at the micro to macro scale in academia and industry, being particularly interesting for pharmaceutical applications. Protein crystallization offers an attractive alternative to chromatography as a downstream processing step in the biotechnology industry, but also in the food and chemical industries. Monitoring of the protein crystallization process is required to understand the crystal growth mechanism and to obtain the information necessary for efficient process control, which needs to comply with the critical quality attributes of the product. Since a wide range of monitoring techniques have already been developed and established, this review focuses on the recent advances of selected techniques in monitoring protein crystallization processes such as the focused beam reflectance method (FBRM), and machine learning-based image analysis for solid-phase monitoring, as well as the spectroscopic methods for liquid-phase monitoring, such as attenuated total reflectance Fourier transform infrared (ATR-FTIR) and UV/Vis spectroscopy.
KW - UV/Vis spectroscopy
KW - attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy
KW - focused beam reflectance measurement (FBRM)
KW - machine learning (ML) based image analysis
KW - monitoring techniques
KW - process analytical technology (PAT)
KW - protein crystallization
UR - http://www.scopus.com/inward/record.url?scp=85160420977&partnerID=8YFLogxK
U2 - 10.3390/cryst13050773
DO - 10.3390/cryst13050773
M3 - Review article
AN - SCOPUS:85160420977
SN - 2073-4352
VL - 13
JO - Crystals
JF - Crystals
IS - 5
M1 - 773
ER -