RBPome identification in egg-cell like callus of Arabidopsis

Liping Liu, Jakob Trendel, Guojing Jiang, Yanhui Liu, Astrid Bruckmann, Bernhard Küster, Stefanie Sprunck, Thomas Dresselhaus, Andrea Bleckmann

Research output: Contribution to journalArticlepeer-review


RNA binding proteins (RBPs) have multiple and essential roles in transcriptional and posttranscriptional regulation of gene expression in all living organisms. Their biochemical identification in the proteome of a given cell or tissue requires significant protein amounts, which limits studies in rare and highly specialized cells. As a consequence, we know almost nothing about the role(s) of RBPs in reproductive processes such as egg cell development, fertilization and early embryogenesis in flowering plants. To systematically identify the RBPome of egg cells in the model plant Arabidopsis, we performed RNA interactome capture (RIC) experiments using the egg cell-like RKD2-callus and were able to identify 728 proteins associated with poly(A+)-RNA. Transcripts for 97% of identified proteins could be verified in the egg cell transcriptome. 46% of identified proteins can be associated with the RNA life cycle. Proteins involved in mRNA binding, RNA processing and metabolism are highly enriched. Compared with the few available RBPome datasets of vegetative plant tissues, we identified 475 egg cell-enriched RBPs, which will now serve as a resource to study RBP function(s) during egg cell development, fertilization and early embryogenesis. First candidates were already identified showing an egg cell-specific expression pattern in ovules.

Original languageEnglish
Pages (from-to)1137-1149
Number of pages13
JournalBiological Chemistry
Issue number11-12
StatePublished - 1 Oct 2023


  • Arabidopsis
  • RKD2-callus
  • RNA binding domains (RBD)
  • RNA binding protein (RBP)
  • RNA interactome capture (RIC)
  • egg cell


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