Rapid procedure for the approximate determination of the deoxyribonucleic acid base composition of Micrococci, Staphylococci and other bacteria

S. A. Meyer, K. H. Schleifer

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A simple procedure for the isolation of DNA is described. The resulting DNA is pure enough to allow a reasonably accurate determination of its base composition by ultraviolet spectroscopy. Since the DNA base composition of micrococci (65 to 75 mol% guanine plus cytosine [G+C]) differs greatly from that of staphylococci (30 to 40 mol% G+C), even an approximate determination of this value is sufficient to distinguish between these two genera.

Original languageEnglish
Pages (from-to)383-385
Number of pages3
JournalInternational Journal of Systematic Bacteriology
Volume25
Issue number4
DOIs
StatePublished - 1975

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