Rapid peptide exchange on MHC class I by small molecules elucidates dynamics of bound peptide

Andries Hadeler, Ankur Saikia, Martin Zacharias, Sebastian Springer

Research output: Contribution to journalArticlepeer-review

Abstract

Complexes of peptides with recombinant major histocompatibility complex class I molecules (rpMHCs) are an important tool for T cell detection, isolation, and activation in cancer immunotherapy. The rapid preparation of rpMHCs is aided by peptide exchange, for which several technologies exist. Here, we show peptide exchange with small-molecule alcohols and demonstrate that they accelerate the dissociation of pre-bound peptides, creating a novel method for rapid production of rpMHCs and increasing the understanding of the conformational flexibility of the MHC-bound peptides.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalCurrent Research in Immunology
Volume3
DOIs
StatePublished - Jan 2022
Externally publishedYes

Keywords

  • Fluorescence anisotropy
  • MHC class I
  • Major histocompatibility complex
  • Nanoscale differential scanning fluorimetry
  • Peptide exchange
  • Small molecule

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