Rapid identification of Escherichia coli safety and laboratory strain lineages based on Multiplex-PCR

Andreas Peter Bauer, Sarah Maria Dieckmann, Wolfgang Ludwig, Karl Heinz Schleifer

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Escherichia coli K-12, B, C and W strains are the most frequently used bacterial safety and laboratory strains. Lineage-specific DNA fragments were detected by microplate subtractive hybridization and utilized to create a fast differentiation method using a single PCR reaction to differentiate clearly the four lineages and separate them from pathogenic variants. The method has been evaluated on a comprehensive selection of widely used laboratory strains and a variety of pathogenic E. coli representatives. In addition, in silico analysis on all available E. coli genomes and the genomes of the close relatives Shigella and Salmonella confirmed the reliability of the proposed method. A fast identification and differentiation of E. coli safety strains by Multiplex-PCR is a useful tool for researchers and companies to check and monitor their reference stocks.

Original languageEnglish
Pages (from-to)36-40
Number of pages5
JournalFEMS Microbiology Letters
Volume269
Issue number1
DOIs
StatePublished - Apr 2007
Externally publishedYes

Keywords

  • Escherichia coli safety strain
  • Identification
  • Multiplex-PCR

Fingerprint

Dive into the research topics of 'Rapid identification of Escherichia coli safety and laboratory strain lineages based on Multiplex-PCR'. Together they form a unique fingerprint.

Cite this