Rapid field screening test for determination of 2,4,6-trinitrotoluene in water and soil with immunofiltration

Claudia Keuchel, Reinhard Niessner

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22 Scopus citations

Abstract

A rapid field screening test for 2,4,6-trinitrotoluene (TNT) in water and soil is described. The immunofiltration assay is based on a simplified enzymelinked immunosorbent assay (ELISA) performed in a pre-packed portable device. Performance of the test was assayed in spiked water, methanolic soil extracts (dilution 1: 10) and natural water samples. A quantitative colour response to concentrations of TNT in the range of 1 to 30 μg/l in water and 50 to 1000 μg/kg in soil is demonstrated. The relative average standard deviations are 11.9% and 14.1%, respectively. High correlations between immunofiltration, ELISA and gas chromatography have been demonstrated. The cross reactivities of the antibody are comparable in both techniques.

Original languageEnglish
Pages (from-to)538-543
Number of pages6
JournalFresenius' Journal of Analytical Chemistry
Volume350
Issue number7-9
DOIs
StatePublished - Jul 1994

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