Rapid discrimination of psychrotolerant and mesophilic strains of the Bacillus cereus group by PCR targeting of 16S rDNA

Felix Von Stetten, Kevin P. Francis, Sabine Lechner, Klaus Neuhaus, Siegfried Scherer

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49 Scopus citations

Abstract

The paper describes a novel PCR assay for discriminating psychrotolerant and mesophilic strains of the Bacillus cereus group by targeting of 16S rDNA signatures. Application of the assay circumvents long-term growth tests at low temperature currently used to detect psychrotolerant strains. PCR was performed with pure cultures. A 100% correlation of PCR and growth data at 7°C was obtained for the 194 B. cereus group strains tested. Potential applications of the assay for the dairy industry and agriculture are suggested. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalJournal of Microbiological Methods
Volume34
Issue number2
DOIs
StatePublished - 1 Nov 1998

Keywords

  • 16S rDNA signature
  • Bacillus cereus
  • Bacillus mycoides
  • Bacillus thuringiensis
  • PCR
  • Psychrotolerant

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