Rapid and simultaneous analysis of 16-O-methylcafestol and sterols as markers for assessment of green coffee bean authenticity by on-line LC-GC

Willibald Kamm; Fabiola Dionisi; Laurent Bernard Fay; Claudia Hischenhuber; Hans Georg Schmarr; Karl Heinz Engel

doi:10.1007/s11746-002-0612-5

Rapid and simultaneous analysis of 16-O-methylcafestol and sterols as markers for assessment of green coffee bean authenticity by on-line LC-GC

Willibald Kamm, Fabiola Dionisi, Laurent Bernard Fay, Claudia Hischenhuber, Hans Georg Schmarr, Karl Heinz Engel

Chair of General Food Technology (2008 — 2024)

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

An on-line LC-GC method for analysis of 16-O-methylcafestol (16-OMC) and total sterols in oils obtained from green beans of Coffea canephora (Robusta) and C. arabica (Arabica) has been developed. HPLC pre-separation of the transesterified lipids allows quantification of the content of 16-OMC. The fraction containing the sterols is transferred to on-line GC analysis. The results obtained for Arabica and Robusta samples were in good agreement with literature data obtained by classical analytical procedures. The method allows rapid and simultaneous determination of two markers (16-OMC and Δ⁵-avenasterol) proposed for authenticity assessment of coffee beans and overcomes time-consuming sample pretreatment steps.

Original language	English
Pages (from-to)	1109-1113
Number of pages	5
Journal	Journal of the American Oil Chemists' Society
Volume	79
Issue number	11
DOIs	https://doi.org/10.1007/s11746-002-0612-5
State	Published - Nov 2002

Keywords

16-O-methylcafestol
Authenticity
Coffea arabica
Coffea robusta
Coffee
Diterpenes
On-line LC-GC
Sterols
Δ-avenasterol

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title = "Rapid and simultaneous analysis of 16-O-methylcafestol and sterols as markers for assessment of green coffee bean authenticity by on-line LC-GC",

abstract = "An on-line LC-GC method for analysis of 16-O-methylcafestol (16-OMC) and total sterols in oils obtained from green beans of Coffea canephora (Robusta) and C. arabica (Arabica) has been developed. HPLC pre-separation of the transesterified lipids allows quantification of the content of 16-OMC. The fraction containing the sterols is transferred to on-line GC analysis. The results obtained for Arabica and Robusta samples were in good agreement with literature data obtained by classical analytical procedures. The method allows rapid and simultaneous determination of two markers (16-OMC and Δ5-avenasterol) proposed for authenticity assessment of coffee beans and overcomes time-consuming sample pretreatment steps.",

keywords = "16-O-methylcafestol, Authenticity, Coffea arabica, Coffea robusta, Coffee, Diterpenes, On-line LC-GC, Sterols, Δ-avenasterol",

author = "Willibald Kamm and Fabiola Dionisi and Fay, {Laurent Bernard} and Claudia Hischenhuber and Schmarr, {Hans Georg} and Engel, {Karl Heinz}",

year = "2002",

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doi = "10.1007/s11746-002-0612-5",

language = "English",

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T1 - Rapid and simultaneous analysis of 16-O-methylcafestol and sterols as markers for assessment of green coffee bean authenticity by on-line LC-GC

AU - Kamm, Willibald

AU - Dionisi, Fabiola

AU - Fay, Laurent Bernard

AU - Hischenhuber, Claudia

AU - Schmarr, Hans Georg

AU - Engel, Karl Heinz

PY - 2002/11

Y1 - 2002/11

N2 - An on-line LC-GC method for analysis of 16-O-methylcafestol (16-OMC) and total sterols in oils obtained from green beans of Coffea canephora (Robusta) and C. arabica (Arabica) has been developed. HPLC pre-separation of the transesterified lipids allows quantification of the content of 16-OMC. The fraction containing the sterols is transferred to on-line GC analysis. The results obtained for Arabica and Robusta samples were in good agreement with literature data obtained by classical analytical procedures. The method allows rapid and simultaneous determination of two markers (16-OMC and Δ5-avenasterol) proposed for authenticity assessment of coffee beans and overcomes time-consuming sample pretreatment steps.

AB - An on-line LC-GC method for analysis of 16-O-methylcafestol (16-OMC) and total sterols in oils obtained from green beans of Coffea canephora (Robusta) and C. arabica (Arabica) has been developed. HPLC pre-separation of the transesterified lipids allows quantification of the content of 16-OMC. The fraction containing the sterols is transferred to on-line GC analysis. The results obtained for Arabica and Robusta samples were in good agreement with literature data obtained by classical analytical procedures. The method allows rapid and simultaneous determination of two markers (16-OMC and Δ5-avenasterol) proposed for authenticity assessment of coffee beans and overcomes time-consuming sample pretreatment steps.

KW - 16-O-methylcafestol

KW - Authenticity

KW - Coffea arabica

KW - Coffea robusta

KW - Coffee

KW - Diterpenes

KW - On-line LC-GC

KW - Sterols

KW - Δ-avenasterol

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U2 - 10.1007/s11746-002-0612-5

DO - 10.1007/s11746-002-0612-5

M3 - Article

AN - SCOPUS:0036852489

SN - 0003-021X

VL - 79

SP - 1109

EP - 1113

JO - Journal of the American Oil Chemists' Society

JF - Journal of the American Oil Chemists' Society

IS - 11

ER -

Rapid and simultaneous analysis of 16-O-methylcafestol and sterols as markers for assessment of green coffee bean authenticity by on-line LC-GC

Abstract

Keywords

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