TY - JOUR
T1 - Quantitative three-dimensional imaging of lipid, protein, and water contents via x-ray phase-contrast tomography
AU - Willner, Marian
AU - Viermetz, Manuel
AU - Marschner, Mathias
AU - Scherer, Kai
AU - Braun, Christian
AU - Fingerle, Alexander
AU - Noël, Peter
AU - Rummeny, Ernst
AU - Pfeiffer, Franz
AU - Herzen, Julia
N1 - Publisher Copyright:
© 2016 Willner et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/3
Y1 - 2016/3
N2 - X-ray phase-contrast computed tomography is an emerging imaging technology with powerful capabilities for three-dimensional (3D) visualization of weakly absorbing objects such as biological soft tissues. This technique is an extension of existing X-ray applications because conventional attenuation-contrast images are simultaneously acquired. The complementary information provided by both the contrast modalities suggests that enhanced material characterization is possible when performing combined data analysis. In this study, we describe how protein, lipid, and water concentrations in each 3D voxel can be quantified by vector decomposition. Experimental results of dairy products, porcine fat and rind, and different human soft tissue types are presented. The results demonstrate the potential of phase-contrast imaging as a new analysis tool. The 3D representations of protein, lipid, and water contents open up new opportunities in the fields of biology, medicine, and food science.
AB - X-ray phase-contrast computed tomography is an emerging imaging technology with powerful capabilities for three-dimensional (3D) visualization of weakly absorbing objects such as biological soft tissues. This technique is an extension of existing X-ray applications because conventional attenuation-contrast images are simultaneously acquired. The complementary information provided by both the contrast modalities suggests that enhanced material characterization is possible when performing combined data analysis. In this study, we describe how protein, lipid, and water concentrations in each 3D voxel can be quantified by vector decomposition. Experimental results of dairy products, porcine fat and rind, and different human soft tissue types are presented. The results demonstrate the potential of phase-contrast imaging as a new analysis tool. The 3D representations of protein, lipid, and water contents open up new opportunities in the fields of biology, medicine, and food science.
UR - http://www.scopus.com/inward/record.url?scp=84962406495&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0151889
DO - 10.1371/journal.pone.0151889
M3 - Article
C2 - 27003308
AN - SCOPUS:84962406495
SN - 1932-6203
VL - 11
JO - PLoS ONE
JF - PLoS ONE
IS - 3
M1 - e0151889
ER -