Quantitative phase-contrast X-ray micro CT for visualization of mouse lymph nodes

Mirko Riedel; Jörg U. Hammel; Julian Moosmann; Felix Beckmann; Florian Schwarzenberg; Paul Schuetz; Stephan Henne; Clemens Wülfing; Madleen Busse; Julia Herzen

doi:10.1117/12.2631184

Quantitative phase-contrast X-ray micro CT for visualization of mouse lymph nodes

Mirko Riedel, Jörg U. Hammel, Julian Moosmann, Felix Beckmann, Florian Schwarzenberg, Paul Schuetz, Stephan Henne, Clemens Wülfing, Madleen Busse, Julia Herzen

Associate Professorship of Physics in Biomedical Imaging

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

Previously, we designed a new quantitative phase-contrast setup for the microtomography experiments at the hereon beamlines P05 and P07 at PETRA III, DESY. This setup is based on Talbot array illuminators (TAI) as high visibility wavefront markers and can reach a spatial resolution level comparable to propagation-based imaging. In this work, we focus on the progress of bringing this setup into user operation. We collaborated with INI-Research to investigate the vascular system of mouse lymph nodes, exploiting the spatial resolution capabilities and using the quantitative aspect of the data to compare different sample preparation methods. We could successfully visualize and trace the blood supply of the lymph nodes, even with fine capillaries, showing the stability and performance of the setup in user application.

Original language	English
Title of host publication	Developments in X-Ray Tomography XIV
Editors	Bert Muller, Ge Wang
Publisher	SPIE
ISBN (Electronic)	9781510654686
DOIs	https://doi.org/10.1117/12.2631184
State	Published - 2022
Event	Developments in X-Ray Tomography XIV 2022 - San Diego, United States Duration: 22 Aug 2022 → 24 Aug 2022

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	12242
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Developments in X-Ray Tomography XIV 2022
Country/Territory	United States
City	San Diego
Period	22/08/22 → 24/08/22

Keywords

Talbot array illuminator
X-ray tomography
lymph nodes
phase-contrast
speckle-based imaging

Access to Document

10.1117/12.2631184

Cite this

Riedel, M., Hammel, J. U., Moosmann, J., Beckmann, F., Schwarzenberg, F., Schuetz, P., Henne, S., Wülfing, C., Busse, M., & Herzen, J. (2022). Quantitative phase-contrast X-ray micro CT for visualization of mouse lymph nodes. In B. Muller, & G. Wang (Eds.), Developments in X-Ray Tomography XIV Article 1224213 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12242). SPIE. https://doi.org/10.1117/12.2631184

@inproceedings{b129e30f20c845998c6e1e77cb33357c,

title = "Quantitative phase-contrast X-ray micro CT for visualization of mouse lymph nodes",

abstract = "Previously, we designed a new quantitative phase-contrast setup for the microtomography experiments at the hereon beamlines P05 and P07 at PETRA III, DESY. This setup is based on Talbot array illuminators (TAI) as high visibility wavefront markers and can reach a spatial resolution level comparable to propagation-based imaging. In this work, we focus on the progress of bringing this setup into user operation. We collaborated with INI-Research to investigate the vascular system of mouse lymph nodes, exploiting the spatial resolution capabilities and using the quantitative aspect of the data to compare different sample preparation methods. We could successfully visualize and trace the blood supply of the lymph nodes, even with fine capillaries, showing the stability and performance of the setup in user application.",

keywords = "Talbot array illuminator, X-ray tomography, lymph nodes, phase-contrast, speckle-based imaging",

author = "Mirko Riedel and Hammel, {J{\"o}rg U.} and Julian Moosmann and Felix Beckmann and Florian Schwarzenberg and Paul Schuetz and Stephan Henne and Clemens W{\"u}lfing and Madleen Busse and Julia Herzen",

note = "Publisher Copyright: {\textcopyright} 2022 SPIE.; Developments in X-Ray Tomography XIV 2022 ; Conference date: 22-08-2022 Through 24-08-2022",

year = "2022",

doi = "10.1117/12.2631184",

language = "English",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE",

editor = "Bert Muller and Ge Wang",

booktitle = "Developments in X-Ray Tomography XIV",

}

Riedel, M, Hammel, JU, Moosmann, J, Beckmann, F, Schwarzenberg, F, Schuetz, P, Henne, S, Wülfing, C, Busse, M & Herzen, J 2022, Quantitative phase-contrast X-ray micro CT for visualization of mouse lymph nodes. in B Muller & G Wang (eds), Developments in X-Ray Tomography XIV., 1224213, Proceedings of SPIE - The International Society for Optical Engineering, vol. 12242, SPIE, Developments in X-Ray Tomography XIV 2022, San Diego, United States, 22/08/22. https://doi.org/10.1117/12.2631184

Quantitative phase-contrast X-ray micro CT for visualization of mouse lymph nodes. / Riedel, Mirko; Hammel, Jörg U.; Moosmann, Julian et al.
Developments in X-Ray Tomography XIV. ed. / Bert Muller; Ge Wang. SPIE, 2022. 1224213 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12242).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Quantitative phase-contrast X-ray micro CT for visualization of mouse lymph nodes

AU - Riedel, Mirko

AU - Hammel, Jörg U.

AU - Moosmann, Julian

AU - Beckmann, Felix

AU - Schwarzenberg, Florian

AU - Schuetz, Paul

AU - Henne, Stephan

AU - Wülfing, Clemens

AU - Busse, Madleen

AU - Herzen, Julia

PY - 2022

Y1 - 2022

N2 - Previously, we designed a new quantitative phase-contrast setup for the microtomography experiments at the hereon beamlines P05 and P07 at PETRA III, DESY. This setup is based on Talbot array illuminators (TAI) as high visibility wavefront markers and can reach a spatial resolution level comparable to propagation-based imaging. In this work, we focus on the progress of bringing this setup into user operation. We collaborated with INI-Research to investigate the vascular system of mouse lymph nodes, exploiting the spatial resolution capabilities and using the quantitative aspect of the data to compare different sample preparation methods. We could successfully visualize and trace the blood supply of the lymph nodes, even with fine capillaries, showing the stability and performance of the setup in user application.

AB - Previously, we designed a new quantitative phase-contrast setup for the microtomography experiments at the hereon beamlines P05 and P07 at PETRA III, DESY. This setup is based on Talbot array illuminators (TAI) as high visibility wavefront markers and can reach a spatial resolution level comparable to propagation-based imaging. In this work, we focus on the progress of bringing this setup into user operation. We collaborated with INI-Research to investigate the vascular system of mouse lymph nodes, exploiting the spatial resolution capabilities and using the quantitative aspect of the data to compare different sample preparation methods. We could successfully visualize and trace the blood supply of the lymph nodes, even with fine capillaries, showing the stability and performance of the setup in user application.

KW - Talbot array illuminator

KW - X-ray tomography

KW - lymph nodes

KW - phase-contrast

KW - speckle-based imaging

UR - http://www.scopus.com/inward/record.url?scp=85184843429&partnerID=8YFLogxK

U2 - 10.1117/12.2631184

DO - 10.1117/12.2631184

M3 - Conference contribution

AN - SCOPUS:85184843429

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Developments in X-Ray Tomography XIV

A2 - Muller, Bert

A2 - Wang, Ge

PB - SPIE

T2 - Developments in X-Ray Tomography XIV 2022

Y2 - 22 August 2022 through 24 August 2022

ER -

Quantitative phase-contrast X-ray micro CT for visualization of mouse lymph nodes

Abstract

Publication series

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Cite this