Quantitative optical coherence tomography imaging of intermediate flow defect phenotypes in ciliary physiology and pathophysiology

Brendan K. Huang, Ute A. Gamm, Stephan Jonas, Mustafa K. Khokha, Michael A. Choma

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Cilia-driven fluid flow is a critical yet poorly understood aspect of pulmonary physiology. Here, we demonstrate that optical coherence tomography-based particle tracking velocimetry can be used to quantify subtle variability in cilia-driven flow performance in Xenopus, an important animal model of ciliary biology. Changes in flow performance werequantified in the setting of normal development, as well as in response to three types of perturbations: mechanical (increased fluid viscosity), pharmacological (disrupted serotonin signaling), and genetic (diminished ciliary motor protein expression). Of note, we demonstrate decreased flow secondary to gene knockdown of kif3a, a protein involved in ciliogenesis, as well as a dose-response decrease in flow secondary to knockdown of dnah9, an important ciliary motor protein.

Original languageEnglish
Article number030502
JournalJournal of Biomedical Optics
Volume20
Issue number3
DOIs
StatePublished - 1 Mar 2015
Externally publishedYes

Keywords

  • Xenopus
  • dnah9
  • kif3a
  • mucus
  • optics
  • particle tracking

Fingerprint

Dive into the research topics of 'Quantitative optical coherence tomography imaging of intermediate flow defect phenotypes in ciliary physiology and pathophysiology'. Together they form a unique fingerprint.

Cite this