Abstract
Cilia-driven fluid flow is a critical yet poorly understood aspect of pulmonary physiology. Here, we demonstrate that optical coherence tomography-based particle tracking velocimetry can be used to quantify subtle variability in cilia-driven flow performance in Xenopus, an important animal model of ciliary biology. Changes in flow performance werequantified in the setting of normal development, as well as in response to three types of perturbations: mechanical (increased fluid viscosity), pharmacological (disrupted serotonin signaling), and genetic (diminished ciliary motor protein expression). Of note, we demonstrate decreased flow secondary to gene knockdown of kif3a, a protein involved in ciliogenesis, as well as a dose-response decrease in flow secondary to knockdown of dnah9, an important ciliary motor protein.
Original language | English |
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Article number | 030502 |
Journal | Journal of Biomedical Optics |
Volume | 20 |
Issue number | 3 |
DOIs | |
State | Published - 1 Mar 2015 |
Externally published | Yes |
Keywords
- Xenopus
- dnah9
- kif3a
- mucus
- optics
- particle tracking