Quantifying telomeric lncRNAs using PNA-labelled RNA-Flow FISH (RNA-Flow)

Iria González-Vasconcellos, María A. Cobos-Fernández, Michael J. Atkinson, José Fernandez-Piqueras, Javier Santos

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Here we present a method to detect and quantify long non-coding RNAs, in particular those related to telomeres. By coupling the specificity of a peptide nucleic acid (PNA) probe with flow cytometry we have quantified cellular levels of TERRA and TERC lncRNAs in culture cell lines and PBMCs. This easy-to-use method appointed RNA-Flow allows reliable lncRNA quantification with broad applications in basic research and clinical diagnostics. In addition, the staining protocol presented here was proven useful for the detection and quantification of such lncRNAs on unfixed cells using confocal microscopy.

Original languageEnglish
Article number513
JournalCommunications Biology
Issue number1
StatePublished - Dec 2022


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