TY - JOUR
T1 - Quantifying telomeric lncRNAs using PNA-labelled RNA-Flow FISH (RNA-Flow)
AU - González-Vasconcellos, Iria
AU - Cobos-Fernández, María A.
AU - Atkinson, Michael J.
AU - Fernandez-Piqueras, José
AU - Santos, Javier
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Here we present a method to detect and quantify long non-coding RNAs, in particular those related to telomeres. By coupling the specificity of a peptide nucleic acid (PNA) probe with flow cytometry we have quantified cellular levels of TERRA and TERC lncRNAs in culture cell lines and PBMCs. This easy-to-use method appointed RNA-Flow allows reliable lncRNA quantification with broad applications in basic research and clinical diagnostics. In addition, the staining protocol presented here was proven useful for the detection and quantification of such lncRNAs on unfixed cells using confocal microscopy.
AB - Here we present a method to detect and quantify long non-coding RNAs, in particular those related to telomeres. By coupling the specificity of a peptide nucleic acid (PNA) probe with flow cytometry we have quantified cellular levels of TERRA and TERC lncRNAs in culture cell lines and PBMCs. This easy-to-use method appointed RNA-Flow allows reliable lncRNA quantification with broad applications in basic research and clinical diagnostics. In addition, the staining protocol presented here was proven useful for the detection and quantification of such lncRNAs on unfixed cells using confocal microscopy.
UR - http://www.scopus.com/inward/record.url?scp=85130689792&partnerID=8YFLogxK
U2 - 10.1038/s42003-022-03452-3
DO - 10.1038/s42003-022-03452-3
M3 - Article
C2 - 35614335
AN - SCOPUS:85130689792
SN - 2399-3642
VL - 5
JO - Communications Biology
JF - Communications Biology
IS - 1
M1 - 513
ER -