Quantification of Proteins and Histone Marks in Drosophila Embryos Reveals Stoichiometric Relationships Impacting Chromatin Regulation

Jacques Bonnet; Rik G.H. Lindeboom; Daniil Pokrovsky; Georg Stricker; Muhammed Hasan Çelik; Ralph A.W. Rupp; Julien Gagneur; Michiel Vermeulen; Axel Imhof; Jürg Müller

doi:10.1016/j.devcel.2019.09.011

Quantification of Proteins and Histone Marks in Drosophila Embryos Reveals Stoichiometric Relationships Impacting Chromatin Regulation

Jacques Bonnet, Rik G.H. Lindeboom, Daniil Pokrovsky, Georg Stricker, Muhammed Hasan Çelik, Ralph A.W. Rupp, Julien Gagneur, Michiel Vermeulen, Axel Imhof, Jürg Müller

Informatics 29 - Chair of Computational Molecular Medicine

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Understanding stoichiometric relationships among relevant components can provide insight into biological systems. Bonnet et al. determine the absolute copy numbers of proteins and histone modifications in Drosophila embryonic nuclei. These data enable a quantitative understanding of how a chromatin state is established and maintained during embryogenesis.

Original language	English
Pages (from-to)	632-644.e6
Journal	Developmental Cell
Volume	51
Issue number	5
DOIs	https://doi.org/10.1016/j.devcel.2019.09.011
State	Published - 2 Dec 2019

Keywords

Drosophila
H3K27 methylation
PRC2
absolute proteome quantification
histone modification quantification

Access to Document

10.1016/j.devcel.2019.09.011

Cite this

Bonnet, J., Lindeboom, R. G. H., Pokrovsky, D., Stricker, G., Çelik, M. H., Rupp, R. A. W., Gagneur, J., Vermeulen, M., Imhof, A., & Müller, J. (2019). Quantification of Proteins and Histone Marks in Drosophila Embryos Reveals Stoichiometric Relationships Impacting Chromatin Regulation. Developmental Cell, 51(5), 632-644.e6. https://doi.org/10.1016/j.devcel.2019.09.011

@article{a24779a06b6e484bbcd5ba39e75f9195,

title = "Quantification of Proteins and Histone Marks in Drosophila Embryos Reveals Stoichiometric Relationships Impacting Chromatin Regulation",

abstract = "Understanding stoichiometric relationships among relevant components can provide insight into biological systems. Bonnet et al. determine the absolute copy numbers of proteins and histone modifications in Drosophila embryonic nuclei. These data enable a quantitative understanding of how a chromatin state is established and maintained during embryogenesis.",

keywords = "Drosophila, H3K27 methylation, PRC2, absolute proteome quantification, histone modification quantification",

author = "Jacques Bonnet and Lindeboom, {Rik G.H.} and Daniil Pokrovsky and Georg Stricker and {\c C}elik, {Muhammed Hasan} and Rupp, {Ralph A.W.} and Julien Gagneur and Michiel Vermeulen and Axel Imhof and J{\"u}rg M{\"u}ller",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

year = "2019",

month = dec,

day = "2",

doi = "10.1016/j.devcel.2019.09.011",

language = "English",

volume = "51",

pages = "632--644.e6",

journal = "Developmental Cell",

issn = "1534-5807",

publisher = "Cell Press",

number = "5",

}

TY - JOUR

T1 - Quantification of Proteins and Histone Marks in Drosophila Embryos Reveals Stoichiometric Relationships Impacting Chromatin Regulation

AU - Bonnet, Jacques

AU - Lindeboom, Rik G.H.

AU - Pokrovsky, Daniil

AU - Stricker, Georg

AU - Çelik, Muhammed Hasan

AU - Rupp, Ralph A.W.

AU - Gagneur, Julien

AU - Vermeulen, Michiel

AU - Imhof, Axel

AU - Müller, Jürg

PY - 2019/12/2

Y1 - 2019/12/2

N2 - Understanding stoichiometric relationships among relevant components can provide insight into biological systems. Bonnet et al. determine the absolute copy numbers of proteins and histone modifications in Drosophila embryonic nuclei. These data enable a quantitative understanding of how a chromatin state is established and maintained during embryogenesis.

AB - Understanding stoichiometric relationships among relevant components can provide insight into biological systems. Bonnet et al. determine the absolute copy numbers of proteins and histone modifications in Drosophila embryonic nuclei. These data enable a quantitative understanding of how a chromatin state is established and maintained during embryogenesis.

KW - Drosophila

KW - H3K27 methylation

KW - PRC2

KW - absolute proteome quantification

KW - histone modification quantification

UR - http://www.scopus.com/inward/record.url?scp=85075456330&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2019.09.011

DO - 10.1016/j.devcel.2019.09.011

M3 - Article

C2 - 31630981

AN - SCOPUS:85075456330

SN - 1534-5807

VL - 51

SP - 632-644.e6

JO - Developmental Cell

JF - Developmental Cell

IS - 5

ER -

Quantification of Proteins and Histone Marks in Drosophila Embryos Reveals Stoichiometric Relationships Impacting Chromatin Regulation

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this