Quantification of micro-organisms in binary mixed populations by Fourier transform infrared (FT-IR) spectroscopy

H. Oberreuter, F. Mertens, H. Seiler, S. Scherer

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Fourier Transform Infrared (FT-IR) spectroscopy was used for the first time to determine the ratios of different microorganisms in mixtures. Exemplarily, systems composed of two food-associated yeast species (Saccharomyces cerevisiae/Hanseniaspora uvarum) and two yoghurt lactic acid bacteria (Lactobacillus acidophilus/Streptococcus salivarius ssp. thermophilus) were investigated. Determination of the cell number ratio in the lactic acid bacteria system was possible with a minimal prediction accuracy of ± 16 ratio percentage points while the minimum accuracy of prediction in the yeast two-component system was ± 4% (both at a 95% confidence level). These results show that FT-IR spectroscopy is potentially a rapid method for the quantification of cell ratios in mixtures of two different microorganisms, provided that the cell ratio does not drop below a certain, system-specific threshold.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalLetters in Applied Microbiology
Volume30
Issue number1
DOIs
StatePublished - 2000

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