Quantification of Fungal Infection in Plant Tissues by Determining the Glucosamine Phenylisothiocyanate Derivative Using HPLC Techniques

Wolfgang F. Osswald; Johannes Jehle; Joachim Firl

doi:10.1016/S0176-1617(11)81760-8

Quantification of Fungal Infection in Plant Tissues by Determining the Glucosamine Phenylisothiocyanate Derivative Using HPLC Techniques

Wolfgang F. Osswald
, Johannes Jehle
, Joachim Firl

Associate Professorship of Pathology of Woody Plants (2008 — 2021)

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

After hydrolysis of chitin from fungal cell walls, the glucosamine released was derivatized with phenylisothiocyanate and was used to quantify fungal infection of different plant tissues. The glucosaminephenylisothiocyanate derivative (GIcN-PiTC) was separated on a reversed-phase HPLC column and quantified afterwards using GlcN-PiTC standards. The advantage of this method is its sensitivity and high reproducibility in a wide range of concentrations.

Original language	English
Pages (from-to)	393-397
Number of pages	5
Journal	Journal of Plant Physiology
Volume	145
Issue number	4
DOIs	https://doi.org/10.1016/S0176-1617(11)81760-8
State	Published - 1995

Keywords

Fungal infection
GalcN
GlcN
HPLC
MeOH
Phenylisothiocyanate
PiTC
galactosamine hydrochloride
glucosamine
glucosamine hydrochloride
methanol
phenylisothiocyanate

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10.1016/S0176-1617(11)81760-8

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title = "Quantification of Fungal Infection in Plant Tissues by Determining the Glucosamine Phenylisothiocyanate Derivative Using HPLC Techniques",

abstract = "After hydrolysis of chitin from fungal cell walls, the glucosamine released was derivatized with phenylisothiocyanate and was used to quantify fungal infection of different plant tissues. The glucosaminephenylisothiocyanate derivative (GIcN-PiTC) was separated on a reversed-phase HPLC column and quantified afterwards using GlcN-PiTC standards. The advantage of this method is its sensitivity and high reproducibility in a wide range of concentrations.",

keywords = "Fungal infection, GalcN, GlcN, HPLC, MeOH, Phenylisothiocyanate, PiTC, galactosamine hydrochloride, glucosamine, glucosamine hydrochloride, methanol, phenylisothiocyanate",

author = "Osswald, \{Wolfgang F.\} and Johannes Jehle and Joachim Firl",

note = "Funding Information: This work was financially supported by the Bayer. Staatsministerium flir Landesentwicklung und Umweltfragen.",

year = "1995",

doi = "10.1016/S0176-1617(11)81760-8",

language = "English",

volume = "145",

pages = "393--397",

journal = "Journal of Plant Physiology",

issn = "0176-1617",

publisher = "Elsevier GmbH",

number = "4",

}

TY - JOUR

T1 - Quantification of Fungal Infection in Plant Tissues by Determining the Glucosamine Phenylisothiocyanate Derivative Using HPLC Techniques

AU - Osswald, Wolfgang F.

AU - Jehle, Johannes

AU - Firl, Joachim

N1 - Funding Information: This work was financially supported by the Bayer. Staatsministerium flir Landesentwicklung und Umweltfragen.

PY - 1995

Y1 - 1995

N2 - After hydrolysis of chitin from fungal cell walls, the glucosamine released was derivatized with phenylisothiocyanate and was used to quantify fungal infection of different plant tissues. The glucosaminephenylisothiocyanate derivative (GIcN-PiTC) was separated on a reversed-phase HPLC column and quantified afterwards using GlcN-PiTC standards. The advantage of this method is its sensitivity and high reproducibility in a wide range of concentrations.

AB - After hydrolysis of chitin from fungal cell walls, the glucosamine released was derivatized with phenylisothiocyanate and was used to quantify fungal infection of different plant tissues. The glucosaminephenylisothiocyanate derivative (GIcN-PiTC) was separated on a reversed-phase HPLC column and quantified afterwards using GlcN-PiTC standards. The advantage of this method is its sensitivity and high reproducibility in a wide range of concentrations.

KW - Fungal infection

KW - GalcN

KW - GlcN

KW - HPLC

KW - MeOH

KW - Phenylisothiocyanate

KW - PiTC

KW - galactosamine hydrochloride

KW - glucosamine

KW - glucosamine hydrochloride

KW - methanol

KW - phenylisothiocyanate

UR - https://www.scopus.com/pages/publications/0029106770

U2 - 10.1016/S0176-1617(11)81760-8

DO - 10.1016/S0176-1617(11)81760-8

M3 - Article

AN - SCOPUS:0029106770

SN - 0176-1617

VL - 145

SP - 393

EP - 397

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

IS - 4

ER -

Quantification of Fungal Infection in Plant Tissues by Determining the Glucosamine Phenylisothiocyanate Derivative Using HPLC Techniques

Abstract

Keywords

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